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Development. 2019 May 2;146(9). pii: dev172502. doi: 10.1242/dev.172502.

The Shh receptor Boc is important for myelin formation and repair.

Author information

1
INSERM-University Paris-Sud/Paris-Saclay; Diseases and Hormones of the Nervous System, U1195, 80 rue du Général Leclerc, F-94276, Le Kremlin-Bicêtre, France.
2
IRCM, Molecular Biology of Neural Development, 110 Pine Avenue West, Montreal, Quebec H2W 1R7, Canada; Department of Medicine, University of Montreal, Montreal, Quebec, Canada; McGill University, Montreal, Quebec, Canada.
3
Institut NeuroMyoGène CNRS UMR 5310-INSERM U1217-Université Claude Bernard Lyon 1, Faculté de Médecine et de Pharmacie 69008 Lyon, France.
4
INSERM-University Paris-Sud/Paris-Saclay; Diseases and Hormones of the Nervous System, U1195, 80 rue du Général Leclerc, F-94276, Le Kremlin-Bicêtre, France elisabeth.traiffort@inserm.fr.

Abstract

Myelination leads to the formation of myelin sheaths surrounding neuronal axons and is crucial for function, plasticity and repair of the central nervous system (CNS). It relies on the interaction of the axons and the oligodendrocytes: the glial cells producing CNS myelin. Here, we have investigated the role of a crucial component of the Sonic hedgehog (Shh) signalling pathway, the co-receptor Boc, in developmental and repairing myelination. During development, Boc mutant mice display a transient decrease in oligodendroglial cell density together with delayed myelination. Despite recovery of oligodendroglial cells at later stages, adult mutants still exhibit a lower production of myelin basic protein correlated with a significant decrease in the calibre of callosal axons and a reduced amount of the neurofilament NF-M. During myelin repair, the altered OPC differentiation observed in the mutant is reminiscent of the phenotype observed after blockade of Shh signalling. In addition, Boc mutant microglia/macrophages unexpectedly exhibit the apparent inability to transition from a highly to a faintly ramified morphology in vivo Altogether, these results identify Boc as an important component of myelin formation and repair.

KEYWORDS:

Axon; Hedgehog signalling; Myelin; Oligodendrocyte

PMID:
31048318
DOI:
10.1242/dev.172502

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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