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Saudi J Gastroenterol. 2019 Apr 29. doi: 10.4103/sjg.SJG_520_18. [Epub ahead of print]

Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells.

Author information

Department of Gastroenterology, The Second People's Hospital of China Three Gorges University, Yichang, P.R. China.
School of Clinical Medical, Hubei University of Chinese Medicine, Wuhan, P.R. China.
Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan, P.R. China.
Department of Gastroenterology, Hubei Provincial Hospital of Traditional Chinese and Western Medicine, Wuhan, P.R. China.
Department of Pulmonary Diseases, Jingmen City Hospital of Traditional Chinese Medicine, Jingmen, P.R. China.
Department of Hepatology, Hubei Provincial Hospital of Chinese Medicine, Wuhan, P.R. China.



This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line.

Materials and Methods:

LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein.


After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01).


BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption.


Bletilla striata polysaccharide; IEC-18 cells; ZO-1; occludin

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