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Saudi J Gastroenterol. 2019 Apr 29. doi: 10.4103/sjg.SJG_520_18. [Epub ahead of print]

Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells.

Author information

1
Department of Gastroenterology, The Second People's Hospital of China Three Gorges University, Yichang, P.R. China.
2
School of Clinical Medical, Hubei University of Chinese Medicine, Wuhan, P.R. China.
3
Department of Infectious Diseases, Renmin Hospital of Wuhan University, Wuhan, P.R. China.
4
Department of Gastroenterology, Hubei Provincial Hospital of Traditional Chinese and Western Medicine, Wuhan, P.R. China.
5
Department of Pulmonary Diseases, Jingmen City Hospital of Traditional Chinese Medicine, Jingmen, P.R. China.
6
Department of Hepatology, Hubei Provincial Hospital of Chinese Medicine, Wuhan, P.R. China.

Abstract

Background/Aims:

This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line.

Materials and Methods:

LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein.

Results:

After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01).

Conclusion:

BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption.

KEYWORDS:

Bletilla striata polysaccharide; IEC-18 cells; ZO-1; occludin

PMID:
31044747
DOI:
10.4103/sjg.SJG_520_18
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