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Chem Commun (Camb). 2019 May 21;55(42):5886-5889. doi: 10.1039/c9cc00144a.

Efficient discovery of novel antimicrobials through integration of synthesis and testing in crude ribosome extract.

Author information

1
State Key Laboratory of Biotherapy and Cancer Center/Collaborative Innovation Center for Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, China. luo_youfu@scu.edu.cn.

Abstract

By coupling in situ [2+3] Huisgen cycloaddition with an in vitro transcription/translation luminescence assay in a crude ribosomal extract, a robust and accurate high-throughput platform was successfully developed and applied for efficient identification of novel structural types of ribosomal inhibitors with antimicrobial activity against drug-resistant bacteria.

PMID:
31041938
DOI:
10.1039/c9cc00144a
[Indexed for MEDLINE]

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