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Future Oncol. 2019 May;15(15):1729-1744. doi: 10.2217/fon-2018-0497. Epub 2019 Apr 30.

MiR-29a inhibits invasion and metastasis of cervical cancer via modulating methylation of tumor suppressor SOCS1.

Author information

1
Department of Obstetrics & Gynecology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
2
Department of Infectious Diseases, The First Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.
3
Department of Medicine & Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR, PR China.
4
Department of Hematology, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, PR China.

Abstract

Aims: To investigate roles of miR-29a-DNMT1-SOCS1 axis in cervical cancer invasion and migration. Materials & methods: The methylation level of SOCS1 was determined by methylation specific PCR. The cell apoptosis, proliferation, migration and invasion were examined by Annexin-V/PI staining, MTT and colony formation assays, plus scratch and transwell assays respectively. The expressions of epithelial-mesenchymal transition and NF-κB related proteins were determined by western blotting. Results: MiR-29a was downregulated, accompanied with DNMT1 upregulation and SOCS1 downregulation in cervical cancer tissues. MiR-29a suppressed DNMT1, inhibited SOCS1 promoter methylation and upregulated its expression. Moreover, miR-29a promoted cell apoptosis, suppressed proliferation, inhibited migration and invasion via inactivation of NF-κB signaling pathway in cervical cancer cells. Conclusion: MiR-29a-DNMT1-SOCS1 axis plays an important role on invasion and metastasis in cervical cancer via NF-κB signaling pathway.

KEYWORDS:

DNMT1; NF-κB; SOCS1; cervical cancer; invasion and metastasis; methylation; miR-29a

PMID:
31038361
DOI:
10.2217/fon-2018-0497

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