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J Sep Sci. 2019 Jul;42(13):2280-2288. doi: 10.1002/jssc.201900051. Epub 2019 May 17.

Study of the peak broadening due to detection in the electrophoretic separation of DNA by CE and microchip CE and the application of image sensor for ultra-small detection cell length.

Author information

1
Institute of Photonics and Bio-medicine, Graduate School of Science, East China University of Science and Technology, Shanghai, P. R. China.
2
Department of Applied Physics, Graduate School of Engineering, Osaka University, Yamadaoka, Suita-city, Osaka, Japan.
3
School of Optoelectronic Engineering, ChangZhou Institute of Technology, Changzhou, Jiangsu, P. R. China.

Abstract

Narrow peaks are important to high-resolution and high-speed separation of DNA fragments by capillary electrophoresis and microchip capillary electrophoresis. Detection cell length is one of the broadening factors, which is often ignored in experiments. However, is it always safe to neglect detection cell length under any condition? To answer this question, we investigated the influence of detection cell length by simulation and experiments. A parameter named as detection cell length ratio was proposed to directly compare the detection cell length and the spatial length of sample band. Electrophoretic peaks generated by various detection cell length ratios were analyzed. A simple rule to evaluate the peak broadening due to detection cell length was obtained. The current states of the detection cell length of detection system and their reliabilities in capillary electrophoresis and microchip capillary electrophoresis were analyzed. Microchip capillary electrophoresis detection with an ultra-small detection cell length of 0.36 μm was easily achieved by using an image sensor.

KEYWORDS:

charge-coupled-device; detection cell length; microchip capillary electrophoresis; peak broadening

PMID:
31038284
DOI:
10.1002/jssc.201900051

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