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[IL-18 and NLRP3 in the Process of Acnevulgaris].

[Article in Chinese]

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Department of Dermatology and Venereology, Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital, Chengdu 610072, China.



To determine the role of inlerleukin-18 (IL-18) and nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) in acne vulgaris.


We used propionibacterium acnes (P.acnes) suspensions [multiplicity of infection (MOI)=0, 10, 20, 30] to stimulate normal human epidermal kerationocytes (NHEKs) for 12, 24, and 36 h, respectively. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the protein level of IL-18. Real-time quantitative PCR (real-time PCR) was adopted to detect the mRNA of IL-18.The NHEKs were divided into three groups: ① siRNA group: NHEKs were pretreated with siRNA for 36 h, followed by 36 h exposure to MOI=30 of P.ances suspensions; ② blank control group: NHEKs free from siRNA transfection and P.ances suspensions; ③ positive control group: NHEKs free from siRNA transfection were exposed to P.ances suspensions (MOI=30).The expression of NLRP3 was detected by Western blot.


The expressions of protein and mRNA of IL-18 increased with exposure to P.ances suspensions in a dose responsive way (r>0.75, P<0.05), with the peak effects showing for MOI=30 at 36 h. The expression of IL-18 decreased in the siRNA group compared with the positive control, but was still higher than that of the blank group( P<0.05).


P.ances stimulates NHEK cells to secrete IL-18. The process possibly requires the involvement of NLRP3.


IL-18 ; NLRP3 ; P.canes


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