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Nat Biomed Eng. 2019 May;3(5):402-413. doi: 10.1038/s41551-019-0393-4. Epub 2019 Apr 29.

Spectral tracing of deuterium for imaging glucose metabolism.

Author information

1
Department of Chemistry, Columbia University, New York, NY, USA.
2
Department of Chemistry, Columbia University, New York, NY, USA. wm2256@columbia.edu.
3
Kavli Institute for Brain Science, Columbia University, New York, NY, USA. wm2256@columbia.edu.

Abstract

Cells and tissues often display pronounced spatial and dynamical metabolic heterogeneity. Common glucose-imaging techniques report glucose uptake or catabolism activity, yet do not trace the functional utilization of glucose-derived anabolic products. Here we report a microscopy technique for the optical imaging, via the spectral tracing of deuterium (STRIDE), of diverse macromolecules derived from glucose. Based on stimulated Raman-scattering imaging, STRIDE visualizes the metabolic dynamics of newly synthesized macromolecules, such as DNA, protein, lipids and glycogen, via the enrichment and distinct spectra of carbon-deuterium bonds transferred from the deuterated glucose precursor. STRIDE can also use spectral differences derived from different glucose isotopologues to visualize temporally separated glucose populations using a pulse-chase protocol. We also show that STRIDE can be used to image glucose metabolism in many mouse tissues, including tumours, brain, intestine and liver, at a detection limit of 10 mM of carbon-deuterium bonds. STRIDE provides a high-resolution and chemically informative assessment of glucose anabolic utilization.

PMID:
31036888
PMCID:
PMC6599680
[Available on 2019-10-29]
DOI:
10.1038/s41551-019-0393-4

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