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Nat Biomed Eng. 2019 May;3(5):402-413. doi: 10.1038/s41551-019-0393-4. Epub 2019 Apr 29.

Spectral tracing of deuterium for imaging glucose metabolism.

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Department of Chemistry, Columbia University, New York, NY, USA.
Department of Chemistry, Columbia University, New York, NY, USA.
Kavli Institute for Brain Science, Columbia University, New York, NY, USA.


Cells and tissues often display pronounced spatial and dynamical metabolic heterogeneity. Common glucose-imaging techniques report glucose uptake or catabolism activity, yet do not trace the functional utilization of glucose-derived anabolic products. Here we report a microscopy technique for the optical imaging, via the spectral tracing of deuterium (STRIDE), of diverse macromolecules derived from glucose. Based on stimulated Raman-scattering imaging, STRIDE visualizes the metabolic dynamics of newly synthesized macromolecules, such as DNA, protein, lipids and glycogen, via the enrichment and distinct spectra of carbon-deuterium bonds transferred from the deuterated glucose precursor. STRIDE can also use spectral differences derived from different glucose isotopologues to visualize temporally separated glucose populations using a pulse-chase protocol. We also show that STRIDE can be used to image glucose metabolism in many mouse tissues, including tumours, brain, intestine and liver, at a detection limit of 10 mM of carbon-deuterium bonds. STRIDE provides a high-resolution and chemically informative assessment of glucose anabolic utilization.

[Available on 2019-10-29]

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