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Biochem Biophys Res Commun. 2019 Jun 18;514(1):323-328. doi: 10.1016/j.bbrc.2019.04.138. Epub 2019 Apr 26.

Exosomal miR-301 derived from mesenchymal stem cells protects myocardial infarction by inhibiting myocardial autophagy.

Author information

1
Department of Cardiology, The Second Hospital of Hebei Medical University, No. 215, Heping West Road, Shijiazhuang City, Hebei Province, 050000, China; Department of Cardiology, Harrision International Peace Hospital, No. 180, Renmin East Road, Hengshui City, Hebei Province, 053000, China.
2
Department of Cardiology, The Second Hospital of Hebei Medical University, No. 215, Heping West Road, Shijiazhuang City, Hebei Province, 050000, China.
3
Department of Cardiology, Harrision International Peace Hospital, No. 180, Renmin East Road, Hengshui City, Hebei Province, 053000, China.
4
Department of Cardiology, The Second Hospital of Hebei Medical University, No. 215, Heping West Road, Shijiazhuang City, Hebei Province, 050000, China. Electronic address: Lyjbs2018@sina.com.

Abstract

PURPOSE:

To investigate the protective effects of miR-301 in exosomes secreted by bone mesenchymal stem cells (BMSCs) on rats' myocardial infarction (MI).

METHODS:

After isolation and culture, BMSCs were identified using flow cytometry. Then exosomes were then isolated. Rats MI models were established and they were divided into 4 groups: Sham group, Model group (injected with PBS), BMSC-Exos group (injected with exosomes secreted by BMSCs), BMSC-301-Exos group (injected with exosomes secreted by BMSCs transfected with miR-301 mimics). Cardiac function was assessed by cardiac echocardiography. Myocardial infarct area was measured by Masson trichrome staining mRNA and proteins expression were measured by qRT-PCR and western blot. Exosome morphology and myocardial cells autophagy were observed by transmission electron microscopy.

RESULTS:

BMSCs were obtained. Rat MI models were successfully established. After rats were injected with exosomes secreted by BMSCs transfected with miR-301 mimics, MI tissues were found to have much higher miR-301 expression, LVEF, LVFS, P62 expression, and remarkably lower LVESD, LVEDD, MI area, LC3-II/LC3-I ratio and autophagosomes numbers compared with BMSC-Exos group (all P < 0.05).

CONCLUSION:

miR-301 in exosomes secreted by BMSCs protected MI by inhibiting myocardial autophagy.

KEYWORDS:

Autophagy; BMSCs; Exosomes; Myocardial infarction; miR-301

PMID:
31036323
DOI:
10.1016/j.bbrc.2019.04.138

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