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Cancers (Basel). 2019 Apr 27;11(5). pii: E591. doi: 10.3390/cancers11050591.

Comprehensive Serum Glycopeptide Spectra Analysis (CSGSA): A Potential New Tool for Early Detection of Ovarian Cancer.

Author information

1
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. hayashimasaru0620@hotmail.com.
2
Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Southern California, Los Angeles, CA 90033, USA. koji.matsuo@med.usc.edu.
3
Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA. koji.matsuo@med.usc.edu.
4
Medical Solution Promotion Department, LSI Medience Corporation, Tokyo 1748555, Japan. tanabe.kazuhiro@mp.medience.co.jp.
5
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. ikedam@tokai-u.jp.
6
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. marikom@tokai-u.jp.
7
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. mm_4amm1302@yahoo.co.jp.
8
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. hiroko.machida@tokai.ac.jp.
9
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. shida@is.icc.u-tokai.ac.jp.
10
Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Kanagawa 2591193, Japan. imanishi@tokai-u.jp.
11
Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, University of Southern California, Los Angeles, CA 90027, USA. brendan.grubbs@med.usc.edu.
12
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. hira@is.icc.u-tokai.ac.jp.
13
Department of Obstetrics and Gynecology, Tokai University School of Medicine, Kanagawa 2591193, Japan. mmikami@is.icc.u-tokai.ac.jp.

Abstract

OBJECTIVES:

To conduct a comprehensive glycopeptide spectra analysis of serum between cancer and non-cancer patients to identify early biomarkers of epithelial ovarian cancer (EOC).

METHODS:

Approximately 30,000 glycopeptide peaks were detected from the digested serum glycoproteins of 39 EOC patients (23 early-stage, 16 advanced-stage) and 45 non-cancer patients (27 leiomyoma and ovarian cyst cases, 18 endometrioma cases) by liquid chromatography mass spectrometry (LC-MS). The differential glycopeptide peak spectra were analyzed to distinguish between cancer and non-cancer groups by employing multivariate analysis including principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and heat maps.

RESULTS:

Examined spectral peaks were filtered down to 2281 serum quantitative glycopeptide signatures for differentiation between ovarian cancer and controls using multivariate analysis. The OPLS-DA model using cross-validation parameters R2 and Q2 and score plots of the serum samples significantly differentiated the EOC group from the non-cancer control group. In addition, women with early-stage clear cell carcinoma and endometriomas were clearly distinguished from each other by OPLS-DA as well as by PCA and heat maps.

CONCLUSIONS:

Our study demonstrates the potential of comprehensive serum glycoprotein analysis as a useful tool for ovarian cancer detection.

KEYWORDS:

comprehensive serum glycopeptide spectra analysis; endometrioma; epithelial ovarian cancer; orthogonal partial square discrimination analysis; ovarian clear cell carcinoma

PMID:
31035594
DOI:
10.3390/cancers11050591
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