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Phytomedicine. 2018 Dec 24;61:152806. doi: 10.1016/j.phymed.2018.12.029. [Epub ahead of print]

Apoptotic effect of Gyejibokryunghwan on uterine sarcoma cells (SK-UT-1B).

Author information

1
Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea.
2
Department of Medical Engineering, Graduate school, Kyung HeeUniversity, Seoul 02447, Korea; Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea.
3
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul 02447, Korea.
4
East-West Medical Research Institute, Kyung Hee University Medical Center, Seoul 02447, Korea.
5
Department of Obstetrics & Gynecology, School of Medicine, Kyung Hee University, Kyung Hee Medical Center, Seoul 02447, Korea.
6
Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea; Department of Obstetrics & Gynecology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
7
Department of Clinical Korean Medicine, Graduate School, Kyung Hee University, Seoul 02447, Korea; East-West Medical Research Institute, Kyung Hee University Medical Center, Seoul 02447, Korea; Department of Obstetrics & Gynecology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea. Electronic address: soulhus@gmail.com.
8
Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul 02447, Korea. Electronic address: moosou94@khu.ac.kr.

Abstract

BACKGROUND:

Diagnosis of uterine sarcomais is a challenging task for clinicians because its position is not easily accessible by current conventional techniques. In addition, standardized treatment for uterine sarcoma has not yet been established due to its rarity and heterogeneity.

HYPOTHESIS/PURPOSE:

We investigated the apoptotic cell death of uterine sarcoma cells (SK-UT-1B) induced by Gyejibokryunghwan (GBH). GBH, an herbal medicine, has been widely used for gynecological diseases in Koean medicine.

METHODS:

SK-UT-1B cells were treated with GBH of varying concentrations from 0 to 500 µg/ml. The mechanism of cell death was investigated through multiple analysis methods, including flow cytometry, cell cycle, and western blotting.

RESULTS:

Flow cytometric analysis revealed that the number of apoptotic cells increased in a GBH dose-dependent manner. The cell populations of sub-G1 and G0/G1 phases were increased by GBH treatment, indicating apoptosisand cell arrest, while the population of S and G2/M phases decreased. With GBH, the expression levels of cleaved caspase-3, -6, and -9 were upregulated, while the expression levels of pro-caspase-3, -6, and -9 were down-regulated in SK-UT-1B cells.

CONCLUSION:

These results are the first observation of uterine sarcoma cell death induced by GBH and confirmation of the mechanism of cell death, which occurred through the intrinsic apoptotic pathway. Clinically, uterine sarcoma has a poor prognosis with no appropriate treatment. GBH may become a new treatment modality for uterine sarcoma.

KEYWORDS:

Apoptosis; Gyejibokryunghwan; Herbal medicine; SK-UT-1B; Uterine sarcoma cells

PMID:
31035046
DOI:
10.1016/j.phymed.2018.12.029
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