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Mol Ecol. 2019 Apr;28(8):1857-1862. doi: 10.1111/mec.15060. Epub 2019 Apr 29.

DNA metabarcoding-Need for robust experimental designs to draw sound ecological conclusions.

Author information

1
Institut de Biologie de l'ENS (IBENS), Département de biologie, Ecole normale supérieure, CNRS, INSERM, Université PSL, Paris, France.
2
Laboratoire d'Ecologie Alpine (LECA), CNRS, Université Grenoble Alpes, Grenoble, France.
3
UiT - The Arctic University of Norway, Tromsø Museum, Tromsø, Norway.
4
Senckenberg Biodiversity and Climate Research Centre, Frankfurt am Main, Germany.
5
LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt, Germany.
6
Department of Nematology, University of California, Riverside, California.
7
Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
8
School of Natural Sciences, Bangor University, Gwynedd, UK.
9
Australian Antarctic Division, Kingston, Tasmania, Australia.
10
School of Biological Sciences, BioProtection Research Centre, University of Canterbury, Christchurch, New Zealand.
11
School of Biological Sciences, University of Essex, Essex, UK.
12
Department of Environmental Science and Policy, Università degli Studi di Milano, Milano, Italy.
13
Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado.
14
Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado.
15
Laboratory for Conservation Biology, Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland.
16
Section for Evolutionary Genomics, Biological Institute, University of Copenhagen, Copenhagen, Denmark.
17
Norwegian University of Science and Technology, University Museum, Trondheim, Norway.
18
Trace and Environmental DNA Laboratory, Department of Environment and Agriculture, Curtin University, Perth, Western Australia, Australia.
19
Environomics Future Science Platform, CSIRO National Collections and Marine Infrastructure, Crawley, Western Australia, Australia.
20
Division of Biology, Kansas State University, Manhattan, Kansas.
21
Section for Genetics and Evolutionary Biology (EVOGENE), University of Oslo, Oslo, Norway.
22
Laboratoire d'Anthropobiologie Moléculaire et d'Imagerie de Synthèse, CNRS UMR 5288, Université de Toulouse, Université Paul Sabatier, Toulouse, France.
23
Lundbeck Foundation GeoGenetics Center, University of Copenhagen, Copenhagen, Denmark.
24
Station Biologique de Roscoff, CNRS UMR 7144, Sorbonne Université, Roscoff, France.
25
CSIRO Ocean & Atmosphere, Lucas Heights, New South Wales, Australia.
26
ID-Gene Ecodiagnostics, Geneva, Switzerland.
27
Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland.
28
Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia.
29
Department of Bioscience, University of Aarhus, Aarhus C, Denmark.
30
Department of Zoology, University of Cambridge, Cambridge, UK.
31
Wellcome Trust Sanger Institute, Cambridge, UK.

KEYWORDS:

data quality; environmental DNA; experimental controls; replication

PMID:
31033079
DOI:
10.1111/mec.15060
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