Send to

Choose Destination
Eur J Oral Sci. 2019 Aug;127(4):340-346. doi: 10.1111/eos.12619. Epub 2019 Apr 29.

Molecular insights into hypomineralized enamel.

Author information

Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Department of Pediatric Dentistry, Institute of Odontology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Swedish NMR Centre, University of Gothenburg, Gothenburg, Sweden.


Hypomineralized enamel may be found in connection with the condition molar incisor hypomineralization (MIH), which has a prevalence of around 15% in most parts of the world. Molar incisor hypomineralization is associated with extensive objective and subjective problems, such as hypersensitivity of the affected teeth, enamel breakdown, and problems with retention of restorations. The etiology behind MIH has not yet been elucidated, but a number of possible factors, which affect the same or different functions of ameloblasts during their different stages of maturation, have been suggested. The aim of this study was to utilize multi-nuclear, solid-state nuclear magnetic resonance (ss-NMR) and time-of-flight secondary ion mass spectroscopy (ToF-SIMS) to elucidate any differences, at a molecular level, between enamel powder prepared from normal, healthy teeth and enamel powder prepared from teeth diagnosed with MIH. 31 P and 23 Na ss-NMR confirmed the presence of HPO 4 2 - and two different Na+ sites in hypomineralized enamel, suggesting a heterogeneous chemical composition. The content of organic components was higher in hypomineralized enamel, as shown by both 13 C ss-NMR and ToF-SIMS, indicating the presence of higher numbers of proteins and phospholipids. The interplay between both is necessary for the formation and mineralization of enamel, which might be disturbed or halted in hypomineralized enamel.


ToF-SIMS; solid-state NMR


Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center