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F1000Res. 2019 Mar 7;8:267. doi: 10.12688/f1000research.17707.1. eCollection 2019.

Efficacy of a silver colloidal gel against selected oral bacteria in vitro.

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Departments of Ophthalmology and Visual Sciences, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Viridis BioPharma Pvt Ltd, Mumbai, Maharashtra, India.
American Biotech Labs LLC, Alpine, Utah, USA.
Selenium Ltd, Austin, TX, USA.


Background: It is necessary to develop new strategies to protect against bacteria such as S treptococcus mutans, S treptococcus sanguis, and Streptococcus salivarius, which contribute to tooth decay and plaque formation. Our current study investigated the efficacy of a colloidal silver gel in inhibiting biofilm formation by these principal oral bacteria , in vitro. The aim of this study was to assess the efficacy of a colloidal silver gel formulation for inhibiting bacterial biofilm formation (Ag-gel) by the principal bacteria that cause plaque formation and tooth decay. Methods: The effect of Ag-gel on viability of S. mutans, S. sanguis, and S. salivarius was assessed by quantifying their colony forming units (CFU) in presence or absence of the test gel. The effect of this formulation on biofilm-forming ability of these bacteria was studied through scanning electron microscopy. Results: Using the CFU assays, over 6 logs of inhibition (100%) were found for S. mutans, S. sanguis, and S. salivarius for the Ag-gel-treated bacteria when compared with the control gel. In addition, the Ag-gel also inhibited biofilm formation by these three bacteria mixed together. These results were confirmed by scanning electron microscopy. Conclusions: The Ag-gel was effective in preventing biofilm formation by S. mutans, S. sanguis, and S. salivarius. This Ag-gel should be tested for the ability to block plaque formation in the mouth, through its use as a tooth paste.


Silver; biofilm; dental caries; dental plaque

Conflict of interest statement

Competing interests: Dr. Dilip Mehta and Dr. Anselm de Souza are directors at Viridis BioPharma Pvt. Ltd. and K W Moeller and C D Moeller are directors at American Biotech Labs.

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