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Stem Cell Reports. 2019 May 14;12(5):1159-1177. doi: 10.1016/j.stemcr.2019.04.001. Epub 2019 Apr 25.

RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells.

Author information

1
INSERM U1051, INM, Hopital Saint Eloi, 80 Avenue Augustin Fliche, 34091 Montpellier, France.
2
Inserm U 1127, CNRS UMR 7225, Sorbonne Universités, UPMC Univ Paris 06 UMR S 1127, Institut du Cerveau et de la Moelle épinière, ICM, 75013, Paris, France.
3
Neuroglial Interactions in Cerebral Physiopathology. CIRB, CNRS UMR 7241/INSERM U1050 Collège de France 11, Place Marcelin Berthelot, 75005 Paris, France.
4
Inserm U 1127, CNRS UMR 7225, Sorbonne Universités, UPMC Univ Paris 06 UMR S 1127, Institut du Cerveau et de la Moelle épinière, ICM, 75013, Paris, France; Department of Neurology, Yale University School of Medicine, New Haven, CT 06511, USA.
5
Institut Pasteur, Department of Developmental and Stem Cell Biology, UMR3738 CNRS, Paris 75015, France.
6
INSERM U1051, INM, Hopital Saint Eloi, 80 Avenue Augustin Fliche, 34091 Montpellier, France; CHU of Montpellier, Hopital Gui de Chaulliac, Pathology Department, 80 Avenue Augustin Fliche, 34091 Montpellier, France.
7
University of Montpellier, Faculté des Sciences, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France.
8
INSERM U1051, INM, Hopital Saint Eloi, 80 Avenue Augustin Fliche, 34091 Montpellier, France; CHU of Montpellier, Hopital Gui de Chaulliac, Neurosurgery Department, 80 Avenue Augustin Fliche, 34091 Montpellier, France.
9
INSERM U1051, INM, Hopital Saint Eloi, 80 Avenue Augustin Fliche, 34091 Montpellier, France; University of Montpellier, Faculté des Sciences, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France. Electronic address: jean-philippe.hugnot@umontpellier.fr.

Abstract

Anamniotes, rodents, and young humans maintain neural stem cells in the ependymal zone (EZ) around the central canal of the spinal cord, representing a possible endogenous source for repair in mammalian lesions. Cell diversity and genes specific for this region are ill defined. A cellular and molecular resource is provided here for the mouse and human EZ based on RNA profiling, immunostaining, and fluorescent transgenic mice. This uncovered the conserved expression of 1,200 genes including 120 transcription factors. Unexpectedly the EZ maintains an embryonic-like dorsal-ventral pattern of expression of spinal cord developmental transcription factors (ARX, FOXA2, MSX1, and PAX6). In mice, dorsal and ventral EZ cells express Vegfr3 and are derived from the embryonic roof and floor plates. The dorsal EZ expresses a high level of Bmp6 and Gdf10 genes and harbors a subpopulation of radial quiescent cells expressing MSX1 and ID4 transcription factors.

KEYWORDS:

Msx1; ependyma; ependymal cells; floor plate; neural stem cells; niche; radial glial cells; regionalization; roof plate; spinal cord; transcription factors

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