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Protoplasma. 2019 Apr 27. doi: 10.1007/s00709-019-01364-4. [Epub ahead of print]

Dynamic changes in histone modification are associated with upregulation of Hsf and rRNA genes during heat stress in maize seedlings.

Author information

1
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
2
School of electrical engineering and Automation, Wuhan University, Wuhan, 430072, China.
3
Institute of Food and Agriculture Standardization, China National Institute of Standardization, Beijing, 100191, China.
4
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China. pwang@mail.hzau.edu.cn.
5
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China. ljli@whu.edu.cn.

Abstract

Histone modification plays a significant role in plant responses to abiotic stress. However, there are little scientific studies available on the involvement of dynamic changes in histone modification in the heat stress response in maize. The present investigation was aimed to analyze the epigenetic mechanisms involved in regulating the physiological and biochemical alterations in maize seedlings under heat stress. Our results and observations indicated an increase in electrolyte leakage and hydrolytic activity of the plasma membrane H+-ATPase as well as the high pigment content and reactive oxygen species (ROS) content under high temperature. Furthermore, decondensation of ribosomal DNA (rDNA) chromatin and a simultaneous increase in rRNA gene expression were observed during heat stress, accompanied by a genome-wide increase in the levels of histone H3K4me2 and H3K9ac. Additionally, chromatin immunoprecipitation (ChIP) analysis revealed that alterations in H3K4me2 and H3K9ac levels occurred in promoter regions, which were found to be associated with the upregulation of heat stress factor (Hsf) and rRNA genes. In conclusion, short-term heat stress induces dynamic histone alterations which are associated with Hsf and rRNA gene transcription, accompanied by perturbations of cell membranes and an increase in ROS during acclimation in maize seedlings.

KEYWORDS:

Heat stress; Heat stress factor (Hsf) gene; Histone modification; Maize; rRNA gene

PMID:
31030267
DOI:
10.1007/s00709-019-01364-4

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