Format

Send to

Choose Destination
Stem Cell Res. 2019 Apr 22;37:101447. doi: 10.1016/j.scr.2019.101447. [Epub ahead of print]

Generation of induced pluripotent stem cells (IBMSi011-A) from a patient with Parkinson's disease carrying LRRK2 p.I1371V mutation.

Author information

1
Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan; Institute of Molecular Medicine, National Taiwan University College of Medicine, Taipei, Taiwan.
2
Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
3
Food Industry Research and development Institute, Hsinchu, Taiwan.
4
Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan. Electronic address: jennifer0820@ibms.sinica.edu.tw.
5
Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan. Electronic address: phsieh@ibms.sinica.edu.tw.
6
Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: chlin@ntu.edu.tw.

Abstract

Leucine rich repeat kinase 2 (LRRK2) is the causative gene for autosomal-dominant familial forms of Parkinson's disease (PD). Here, we generated induced pluripotent stem cells (iPSCs) from the peripheral blood mononuclear cells of a female patient with LRRK2 c.4111A > G (p.I1371V) mutation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype. The iPSCs also showed pluripotency confirmed by immunofluorescent staining and differentiated into the three germ layers in vivo. This cellular model will provide a platform for studying the role of LRRK2 in the disease process.

PMID:
31029016
DOI:
10.1016/j.scr.2019.101447
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center