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Angew Chem Int Ed Engl. 2019 Jun 17;58(25):8426-8431. doi: 10.1002/anie.201902537. Epub 2019 May 14.

Development of a Universal Fluorescent Probe for Gram-Positive Bacteria.

Author information

1
Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, Gyeongbuk, 37673, Korea.
2
Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 37673, Korea.
3
Department of Chemistry, Life Sciences Institute, National University of Singapore, 3 Science Drive 3, 117543, Singapore, Singapore.
4
New Drug Discovery Center, Daegu-Gyeongbuk Medivalley Innovation Foundation (DGMIF), 80 Chumbok-ro, Dong-Gu, Daegu, 41061, Korea.
5
SL VAXiGEN A-B1, Korea Bio Park, Seongnam-si, 134488, Gyenggi-do, Korea.
6
Department of Chemical Biology, Max-Planck-Institute for Molecular Physiology, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany.
7
Singapore Bioimaging Consortium, Agency for Science, Technology and Research, 11 Biopolis Way, # 02-02 Helios, 138667, Singapore, Singapore).

Abstract

The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram-positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram-positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application.

KEYWORDS:

Gram-positive bacteria; bioimaging; boronic acid; fluorescent probes; structure-activity relationships

PMID:
31025486
DOI:
10.1002/anie.201902537

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