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Plant Cell. 2019 Jul;31(7):1430-1445. doi: 10.1105/tpc.19.00132. Epub 2019 Apr 25.

Leaf Variegation and Impaired Chloroplast Development Caused by a Truncated CCT Domain Gene in albostrians Barley.

Author information

1
Genomics of Genetic Resources Group, Department of Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Seeland, Germany.
2
Molecular Genetics Group, Institute of Biology, Humboldt University, 10115 Berlin, Germany.
3
Plant Reproductive Biology Group, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Seeland, Germany.
4
Domestication Genomics Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Seeland, Germany.
5
Structural Cell Biology Group, Department of Physiology and Cell Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Seeland, Germany.
6
Bioinformatics and Information Technology Group, Department of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Seeland, Germany.
7
KWS LOCHOW, 29303 Bergen, Germany.
8
Molecular Genetics Group, Institute of Biology, Humboldt University, 10115 Berlin, Germany thomas.boerner@rz.hu-berlin.de stein@ipk-gatersleben.de.
9
Genomics of Genetic Resources Group, Department of Genebank, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Seeland, Germany thomas.boerner@rz.hu-berlin.de stein@ipk-gatersleben.de.

Abstract

Chloroplasts fuel plant development and growth by converting solar energy into chemical energy. They mature from proplastids through the concerted action of genes in both the organellar and the nuclear genome. Defects in such genes impair chloroplast development and may lead to pigment-deficient seedlings or seedlings with variegated leaves. Such mutants are instrumental as tools for dissecting genetic factors underlying the mechanisms involved in chloroplast biogenesis. Characterization of the green-white variegated albostrians mutant of barley (Hordeum vulgare) has greatly broadened the field of chloroplast biology, including the discovery of retrograde signaling. Here, we report identification of the ALBOSTRIANS gene HvAST (also known as Hordeum vulgare CCT Motif Family gene 7, HvCMF7) by positional cloning as well as its functional validation based on independently induced mutants by Targeting Induced Local Lesions in Genomes (TILLING) and RNA-guided clustered regularly interspaced short palindromic repeats-associated protein 9 endonuclease-mediated gene editing. The phenotypes of the independent HvAST mutants imply residual activity of HvCMF7 in the original albostrians allele conferring an imperfect penetrance of the variegated phenotype even at homozygous state of the mutation. HvCMF7 is a homolog of the Arabidopsis (Arabidopsis thaliana) CONSTANS, CO-like, and TOC1 (CCT) Motif transcription factor gene CHLOROPLAST IMPORT APPARATUS2, which was reported to be involved in the expression of nuclear genes essential for chloroplast biogenesis. Notably, in barley we localized HvCMF7 to the chloroplast, without any clear evidence for nuclear localization.

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