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Int Urol Nephrol. 2019 Jul;51(7):1207-1218. doi: 10.1007/s11255-019-02113-5. Epub 2019 Apr 24.

Intervention of mitochondrial activity attenuates cisplatin-induced acute kidney injury.

Yang Y1,2,3, Fu Y1,2,3, Wang P1,2,3, Liu S1,2,3, Sha Y1,2,3, Zhang Y1,2,3, Zhang A1,2,3, Jia Z1,2,3, Ding G4,5,6, Huang S7,8,9.

Author information

1
Department of Nephrology, Children's Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing, 210008, China.
2
Jiangsu Key Laboratory of Pediatrics, Nanjing Medical University, Nanjing, 210029, China.
3
Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China.
4
Department of Nephrology, Children's Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing, 210008, China. bhgyuan@163.com.
5
Jiangsu Key Laboratory of Pediatrics, Nanjing Medical University, Nanjing, 210029, China. bhgyuan@163.com.
6
Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. bhgyuan@163.com.
7
Department of Nephrology, Children's Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing, 210008, China. smhuang@njmu.edu.cn.
8
Jiangsu Key Laboratory of Pediatrics, Nanjing Medical University, Nanjing, 210029, China. smhuang@njmu.edu.cn.
9
Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China. smhuang@njmu.edu.cn.

Abstract

OBJECTIVES:

The dysfunction of mitochondrial respiratory chain induced by cisplatin results in overproduction of reactive oxygen species (ROS) which contributes to kidney injury. The current study aimed to evaluate the effect of a mitochondrial electron transport inhibitors of rotenone (mitochondrial complex I inhibitor) and azoxystrobin (mitochondrial complex III inhibitor), in cisplatin-induced kidney injury.

METHODS:

In vivo, cisplatin was administered to male C57BL/6J mice by a single intraperitoneal (i.p.) injection (20 mg/kg). Then the mice were treated with or without 200 ppm rotenone in food. Mice were sacrificed after cisplatin administration for 72 h. The serum and the kidney tissues were collected for further analysis. In vitro, mouse proximal tubular cells (mPTCs) were treated with cisplatin (5 µg/mL) and rotenone/azoxystrobin for 24 h. Flow cytometry, Western blotting, and TUNEL staining were used to evaluate the cell injury.

RESULTS:

In vivo, rotenone treatment obviously ameliorated cisplatin-induced renal tubular injury evidenced by the improved histology and blocked NGAL upregulation. Meanwhile, cisplatin-induced renal dysfunction shown by the increased levels of serum creatinine (Scr), blood urea nitrogen (BUN), and cystatin C were significantly reduced by rotenone treatment. Moreover, the increments of cleaved caspase-3 and transferase dUTP nick-end labeling (TUNEL)-positive cells were markedly decreased in line with the attenuated mitochondrial dysfunction and oxidative stress after rotenone administration. In vitro, rotenone and azoxystrobin protected against mitochondrial dysfunction, oxidative stress, and renal tubular cell apoptosis induced by cisplatin.

CONCLUSIONS:

Our results demonstrated that inhibition of mitochondrial activity significantly attenuated cisplatin nephrotoxicity possibly by inhibiting mitochondrial oxidative stress.

KEYWORDS:

AKI; Cisplatin; Mitochondrial activity; Oxidative stress

PMID:
31020626
DOI:
10.1007/s11255-019-02113-5

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