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J Antibiot (Tokyo). 2019 Aug;72(8):640-644. doi: 10.1038/s41429-019-0185-9. Epub 2019 Apr 24.

Molecular targets of biofabricated silver nanoparticles in Candida albicans.

Author information

1
School of Life Sciences, SRTM University, Nanded, Maharashtra, 431606, India.
2
Department of Stem Cell and Regenerative medicine, Centre For Interdisciplinary Research, DY Patil Education Society (Deemed to be University), Kolhapur, Maharashtra, 416006, India.
3
Department of Biotechnology, Savitribai Phule, Pune University, Ganeshkhind, Pune, Maharashtra, 411007, India.
4
Department of Technology, Savitribai Phule, Pune University, Ganeshkhind, Pune, Maharashtra, 411007, India.
5
School of Life Sciences, SRTM University, Nanded, Maharashtra, 431606, India. prof.karuppayil@gmail.com.

Abstract

We have analyzed the expressions of genes which regulate Ras-cAMP-EFG1 and CEK1-MAPK pathways involved in yeast to hyphal form morphogenesis in Candida albicans. The expression profile of genes associated with serum-induced morphogenesis showed reduced expressions of genes involved in these pathways by the treatment with biofabricated silver nanoparticles. Cell elongation gene, ECE1, was downregulated by 5.1 fold by the treatment of silver nanoparticles. Expression of hyphal inducer gene, TEC1 was downregulated by 6.28 fold. Negative regulators of yeast to hyphal transition, TUP1 and RFG1 were downregulated by 2.45 and 5.43 fold, respectively. Current study suggests that silver nanoparticles affect gene expression and may subsequently reduce virulence in C. albicans. Targeting genes involved in virulence may be an acceptable novel treatment strategy for pathogenic fungal infections.

PMID:
31019258
DOI:
10.1038/s41429-019-0185-9

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