Chromagar™ requires secondary confirmation strategies to minimize false positive/negative results for detection of Staphylococcus aureus

J Microbiol Methods. 2019 Jun:161:71-73. doi: 10.1016/j.mimet.2019.04.013. Epub 2019 Apr 20.

Abstract

Chromagar is a medium that is used in culture-based colonization studies of Staphylococcus aureus. We have found that S. aureus negative colonies often fit the color recommendations for S. aureus identification and can cause overestimation of colonization rates. Confirmation of suspect colonies is important to minimize false negative/positive results.

Keywords: Chromagar; Colonization; Staphylococcus aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriological Techniques / methods*
  • Chromogenic Compounds*
  • Culture Media
  • Diagnostic Tests, Routine / methods
  • False Negative Reactions
  • False Positive Reactions
  • Humans
  • Negative Results
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / isolation & purification*

Substances

  • Chromogenic Compounds
  • Culture Media