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Vet Parasitol Reg Stud Reports. 2018 Dec;14:170-175. doi: 10.1016/j.vprsr.2018.10.005. Epub 2018 Oct 29.

Serological and molecular diagnosis of Ehrlichia canis and associated risk factors in dogs domiciled in western Cuba.

Author information

1
Department of Animal Prevention, Veterinary Medicine College, Agrarian University of Habana, Mayabeque, Cuba. Electronic address: maylingo@unah.edu.cu.
2
Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
3
Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil. Electronic address: carlosmassard@ufrrj.br.
4
Department of Animal Prevention, Veterinary Medicine College, Agrarian University of Habana, Mayabeque, Cuba. Electronic address: roque@unah.edu.cu.
5
Department of Animal Prevention, Veterinary Medicine College, Agrarian University of Habana, Mayabeque, Cuba. Electronic address: jcesar@unah.edu.cu.
6
National Center for Animal and Plant Health, Mayabeque, Cuba.
7
Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil. Electronic address: adivaldofonseca@yahoo.com.

Abstract

Ehrlichia canis is a rickettsia transmitted by the tick, Rhipicephalus sanguineus, and is the causative agent of canine monocytic ehrlichiosis (CME). In Cuba, the first diagnosis of CME was made in 2001, but few studies have since investigated this disease locally. The objective of this study was to estimate the prevalence of E. canis in dogs domiciled in four municipalities within the western region of Cuba and determine the associated risk factors. Blood was drawn from 378 selected dogs living in four municipalities in two provinces of western Cuba. From the total number of samples, 206 plasma samples were selected to perform an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against E. canis. Using the original 378 samples of extracted DNA, a nested polymerase chain reaction (nPCR) was performed to amplify a specific fragment of the 16S rRNA gene of E. canis. Analysis of the 206 plasma samples revealed a total of 162 animals that were seropositive for E. canis (78.64%) with a density index between 109.5 and 970.7. In contrast, 179 samples were positive based on the nPCR assay (47.35%). As well, there was a high concordance (kappa = 0.7), calculated through the Kappa index, between the animals found to be positive based on nPCR and those determined based on ELISA. The analysis of risk factors showed that residing in the municipality of Boyeros in addition to having a history of infestation by ticks increases the probability of having a positive result based on nPCR.

KEYWORDS:

Diagnosis; Domestic dogs; Ehrlichia canis; Risk factors

PMID:
31014724
DOI:
10.1016/j.vprsr.2018.10.005
[Indexed for MEDLINE]

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