Format

Send to

Choose Destination
J R Soc Interface. 2019 Apr 26;16(153):20180935. doi: 10.1098/rsif.2018.0935.

Growing from a few cells: combined effects of initial stochasticity and cell-to-cell variability.

Author information

1
1 LadHyX and Department of Mechanics, Ecole Polytechnique, CNRS , 91128 Palaiseau , France.
2
2 Department Genomes and Genetics, Physical microfluidics and Bioengineering, Institut Pasteur , 75015 Paris , France.
3
3 Center for Research and Interdisciplinarity , 75014 Paris , France.

Abstract

The growth of a cell population from a large inoculum appears deterministic, although the division process is stochastic at the single-cell level. Microfluidic observations, however, display wide variations in the growth of small populations. Here we combine theory, simulations and experiments to explore the link between single-cell stochasticity and the growth of a population starting from a small number of individuals. The study yields descriptors of the probability distribution function (PDF) of the population size under three sources of stochasticity: cell-to-cell variability, uncertainty in the number of initial cells and generation-dependent division times. The PDF, rescaled to account for the exponential growth of the population, is found to converge to a stationary distribution. All moments of the PDF grow exponentially with the same growth rate, which depends solely on cell-to-cell variability. The shape of the PDF, however, contains the signature of all sources of stochasticity, and is dominated by the early stages of growth, and not by the cell-to-cell variability. Thus, probabilistic predictions of the growth of bacterial populations can be obtained with implications for both naturally occurring conditions and technological applications of single-cell microfluidics.

KEYWORDS:

microbiology; microfluidics; stochastic population dynamics

PMID:
31014203
PMCID:
PMC6505559
[Available on 2020-04-01]
DOI:
10.1098/rsif.2018.0935

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center