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Plant Methods. 2019 Apr 13;15:36. doi: 10.1186/s13007-019-0421-0. eCollection 2019.

In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars.

Author information

1Department of Horticultural Science, Kyungpook National University, Daegu, 4165122 South Korea.
Department of Agricultural Education, Suncheon National University, Suncheon, South Korea.
2School of Applied Biosciences, Kyungpook National University, Daegu, South Korea.
Contributed equally



As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the production of true-to-type plants of five different strawberry cultivars from meristems cultured in media containing different concentrations of kinetin (Kn).


For all the cultivars, shoot induction was successful only in the meristems cultured in the medium without Kn and the medium containing 0.5 mg L-1 Kn. The shoots obtained from explants cultured in media supplemented with 0.5 mg L-1 Kn exhibited better plant growth parameters than those cultured in media without Kn and were genetically stable when compared with conventionally propagated plants for all the cultivars. Vegetative and sexual characters and fruit quality attributes observed in the plants derived from meristems cultured on 0.5 mg L-1 Kn and the conventionally propagated plants were not significantly different when grown for three continuous growing seasons under greenhouse conditions.


The culture of meristems in the medium containing 0.5 mg L-1 Kn is suitable for the efficient propagation of true-to-type plants of different strawberry cultivars and continuous production of fruits with stable quality. Hence, we expect that the method presented in this study will be helpful for the commercial production of true-to-type plants generated in vitro for other strawberry cultivars.


Field performance; Fruit quality; Genetic variation; Kinetin; Meristem

Conflict of interest statement

The authors declare that they have no competing interests.

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