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Viruses. 2019 Apr 19;11(4). pii: E360. doi: 10.3390/v11040360.

First Evidence of Antibodies Against Lloviu Virus in Schreiber's Bent-Winged Insectivorous Bats Demonstrate a Wide Circulation of the Virus in Spain.

Author information

1
Arbovirus and Imported Viral Diseases Laboratory, National Centre of Microbiology, Majadahonda, 28220 Madrid, Spain. erarellano75@gmail.com.
2
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA. mariano.sanchez-lockhart.ctr@mail.mil.
3
Department of Pathology & Microbiology, University of Nebraska Medical Centre, Omaha, NE 68198, USA. mariano.sanchez-lockhart.ctr@mail.mil.
4
National Centre of Microbiology, Parasitology Section, Majadahonda, 28220 Madrid, Spain. chus.perteguer@isciii.es.
5
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA. Maggie.l.bartlett.ctr@mail.mil.
6
Department of Pathology & Microbiology, University of Nebraska Medical Centre, Omaha, NE 68198, USA. Maggie.l.bartlett.ctr@mail.mil.
7
National Centre of Microbiology, Institute of Health Carlos III, Majadahonda, 28220 Madrid, Spain. mortiz@isciii.es.
8
National Centre of Microbiology, Parasitology Section, Majadahonda, 28220 Madrid, Spain. plcampioli@isciii.es.
9
National Centre of Microbiology, Parasitology Section, Majadahonda, 28220 Madrid, Spain. ahergon@isciii.es.
10
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA. jcgonzalezsantos@gmail.com.
11
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA. Karla.y.garcia2.ctr@mail.mil.
12
Department of Pathology & Microbiology, University of Nebraska Medical Centre, Omaha, NE 68198, USA. Karla.y.garcia2.ctr@mail.mil.
13
National Centre of Microbiology, Viral Immunology Laboratory, Majadahonda, 28220 Madrid, Spain. mbuylla@cbm.csic.es.
14
Centro de Investigación en Sanidad Animal-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CISA), 28040 Madrid, Spain. majimenez@inia.es.
15
Centro de Investigación Biomédica en Red de Epidemiologia y Salud Pública (CIBERESP), 28029 Madrid, Spain. majimenez@inia.es.
16
Arbovirus and Imported Viral Diseases Laboratory, National Centre of Microbiology, Majadahonda, 28220 Madrid, Spain. antonio.tenorio.matanzo@gmail.com.
17
Arbovirus and Imported Viral Diseases Laboratory, National Centre of Microbiology, Majadahonda, 28220 Madrid, Spain. paz.sanchez@isciii.es.
18
Museo de la Naturaleza de Cantabria, Carrejo, 39592 Cantabria, Spain. fei2@ctv.es.
19
Centro de Investigación Biomédica en Red de Epidemiologia y Salud Pública (CIBERESP), 28029 Madrid, Spain. jeecheva@isciii.es.
20
National Centre of Microbiology, Virology Section, Majadahonda, 28220 Madrid, Spain. jeecheva@isciii.es.
21
Centre for Genome Sciences, U.S. Army Medical Research Institute of Infectious Diseases, USAMRIID, Fort Detrick, Frederick, MD 21702, USA. gustavo.f.palacios.ctr@mail.mil.
22
Arbovirus and Imported Viral Diseases Laboratory, National Centre of Microbiology, Majadahonda, 28220 Madrid, Spain. anabelnegredo@isciii.es.

Abstract

Although Lloviu virus (LLOV) was discovered in the carcasses of insectivorous Schreiber's Bent-winged bats in the caves of Northern Spain in 2002, its infectivity and pathogenicity remain unclear. We examined the seroprevalence of LLOV in potentially exposed Schreiber's Bent-winged bats (n = 60), common serotine bats (n = 10) as controls, and humans (n = 22) using an immunoblot assay. We found antibodies against LLOV GP2 in all of Schreiber's Bent-winged bats serum pools, but not in any of the common serotine bats and human pools tested. To confirm this seroreactivity, 52 serums were individually tested using Domain Programmable Arrays (DPA), a phage display based-system serology technique for profiling filovirus epitopes. A serological signature against different LLOV proteins was obtained in 19/52 samples tested (36.5%). The immunodominant response was in the majority specific to LLOV-unique epitopes, confirming that the serological response detected was to LLOV. To our knowledge, this is the first serological evidence of LLOV exposure in live captured Schreiber's Bent-winged bats, dissociating LLOV circulation as the cause of the previously reported die-offs.

KEYWORDS:

Bats; Human; Lloviu virus; Prevalence; Serology

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