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J Res Med Sci. 2019 Mar 25;24:26. doi: 10.4103/jrms.JRMS_129_17. eCollection 2019.

Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484.

Author information

1
Department of Ultrasound, Chinese People's Liberation Army General Hospital, Beijing, China.
2
Department of Oncology, Suizhou Hospital, Hubei University of Medicine, Suizhou, China.
3
Department of Liver Disease, Army General Hospital of PLA, Beijing, China.

Abstract

Background:

The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells.

Materials and Methods:

Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay after 72 h. Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR.

Results:

With the treatment of TNF484, the cell proliferation of HepG2 and Bel7402 cells was inhibited in a dose-dependent manner. Moreover, under TNF484 treatment, the cell migration rate as well as cell invasion ability of the HepG2 and Bel7402 cells were suppressed.

Conclusion:

TNF484 could inhibit the cell proliferation, migration, and invasion of some HCC cell lines, making it a potential therapeutic option for liver cancer treatment.

KEYWORDS:

ADAM17; TNF484; hepatocellular carcinoma

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