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J Res Med Sci. 2019 Mar 25;24:26. doi: 10.4103/jrms.JRMS_129_17. eCollection 2019.

Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484.

Author information

Department of Ultrasound, Chinese People's Liberation Army General Hospital, Beijing, China.
Department of Oncology, Suizhou Hospital, Hubei University of Medicine, Suizhou, China.
Department of Liver Disease, Army General Hospital of PLA, Beijing, China.



The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells.

Materials and Methods:

Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay after 72 h. Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR.


With the treatment of TNF484, the cell proliferation of HepG2 and Bel7402 cells was inhibited in a dose-dependent manner. Moreover, under TNF484 treatment, the cell migration rate as well as cell invasion ability of the HepG2 and Bel7402 cells were suppressed.


TNF484 could inhibit the cell proliferation, migration, and invasion of some HCC cell lines, making it a potential therapeutic option for liver cancer treatment.


ADAM17; TNF484; hepatocellular carcinoma

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