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J Environ Sci Health A Tox Hazard Subst Environ Eng. 2019;54(9):865-873. doi: 10.1080/10934529.2019.1603746. Epub 2019 Apr 20.

Biochanin A prevents 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced adipocyte dysfunction in cultured 3T3-L1 cells.

Author information

1
a Department of Endocrinology & Metabolism, School of Medicine , Kyung Hee University , Seoul , Republic of Korea.
2
b Department of Medicine, Graduate School , Kyung Hee University , Seoul , Republic of Korea.
3
c Department of Endocrinology & Metabolism , Kyung Hee University Hospital , Seoul , Republic of Korea.

Abstract

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent environmental pollutant. TCDD accumulates in the food chain, mainly in the fatty tissues of the human body where it causes various toxic effects. Biochanin A is a natural organic compound in the class of phytochemicals known as flavonoids. We investigated whether biochanin A suppresses TCDD-induced loss of adipogenic action using 3T3-L1 adipocytes as a cell culture model of wasting syndrome. In the present study, biochanin A suppressed TCDD-induced loss of lipid accumulation. Pretreating the cells with biochanin A increased the levels of the adipogenesis-associated factors peroxisome proliferator-activated receptor γ and adiponectin, which were inhibited by TCDD. TCDD decreased insulin-stimulated glucose uptake, which was effectively restored by pretreatment with biochanin A. Biochanin A also inhibited the TCDD-driven decrease in production of insulin receptor substrate-1 and glucose transporter 4. These results suggest a preventive effect of biochanin A against TCDD in the development of insulin resistance and diabetes. TCDD increased production of intracellular calcium ([Ca2+]i), prostaglandin E2, cytosolic phospholipase A2, and cyclooxygenase-1, while reducing the level of peroxisome proliferator-activated receptor gamma coactivator 1-alpha. However, biochanin A inhibited these TCDD-induced effects. We conclude that biochanin A is an attractive compound for preventing TCDD-induced wasting syndrome.

KEYWORDS:

2,3,7,8-tetrachlorodibenzo-p-dioxin; 3T3-L1 adipocytes; Biochanin A; differentiation

PMID:
31007129
DOI:
10.1080/10934529.2019.1603746
[Indexed for MEDLINE]

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