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Nat Commun. 2019 Apr 18;10(1):1809. doi: 10.1038/s41467-019-09803-9.

ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer.

Author information

1
Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
2
Swiss Institute of Bioinformatics (SIB), CH-1015, Lausanne, Switzerland.
3
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234, Moscow, Russian Federation.
4
Immunogenomics & Systems Biology group, Institute of Life Sciences, Bhubaneswar, 751023, Odisha, India.
5
Laboratory of Virology and Genetics, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.
6
Institute of Food Nutrition and Health, Eidgenössische Technische Hochschule Zürich (ETHZ), CH-8603, Schwerzenbach, Switzerland.
7
Laboratory of Systems Biology and Genetics, Institute of Bioengineering, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland. bart.deplancke@epfl.ch.
8
Swiss Institute of Bioinformatics (SIB), CH-1015, Lausanne, Switzerland. bart.deplancke@epfl.ch.

Abstract

Krüppel-associated box zinc finger proteins (KZFPs) constitute the largest family of mammalian transcription factors, but most remain completely uncharacterized. While initially proposed to primarily repress transposable elements, recent reports have revealed that KFZPs contribute to a wide variety of other biological processes. Using murine and human in vitro and in vivo models, we demonstrate here that one poorly studied KZFP, ZFP30, promotes adipogenesis by directly targeting and activating a retrotransposon-derived Pparg2 enhancer. Through mechanistic studies, we further show that ZFP30 recruits the co-regulator KRAB-associated protein 1 (KAP1), which, surprisingly, acts as a ZFP30 co-activator in this adipogenic context. Our findings provide an understanding of both adipogenic and KZFP-KAP1 complex-mediated gene regulation, showing that the KZFP-KAP1 axis can also function in a non-repressive manner.

PMID:
31000713
PMCID:
PMC6472429
DOI:
10.1038/s41467-019-09803-9
[Indexed for MEDLINE]
Free PMC Article

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