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Nutrients. 2019 Apr 17;11(4). pii: E869. doi: 10.3390/nu11040869.

Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle.

Author information

1
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. yohno@sozo.ac.jp.
2
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. r1482701@sc.sozo.ac.jp.
3
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. r1482703@sc.sozo.ac.jp.
4
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. r1482716@sc.sozo.ac.jp.
5
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. r1482724@sc.sozo.ac.jp.
6
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. r1382605@sc.sozo.ac.jp.
7
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. r1382606@sc.sozo.ac.jp.
8
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. s-yokoyama@sozo.ac.jp.
9
Laboratory of Sports and Exercise Medicine, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan. egawa.tatsuro.4u@kyoto-u.ac.jp.
10
Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. gotok@sepia.ocn.ne.jp.
11
Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, Toyohashi 440-8511, Japan. gotok@sepia.ocn.ne.jp.

Abstract

The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injected (CX), and (4) lactate-administered after CTX-injection (LX) groups. CTX was injected into right tibialis anterior (TA) muscle before the oral administration of sodium lactate (five days/week for two weeks) to the mice. Oral lactate administration increased the muscle weight and fiber cross-sectional area, and the population of Pax7-positive nuclei in mouse TA skeletal muscle. Oral administration of lactate also facilitated the recovery process of CTX-associated injured mouse TA muscle mass accompanied with a transient increase in the population of Pax7-positive nuclei. Mouse myoblast-derived C2C12 cells were differentiated for five days to form myotubes with or without lactate administration. C2C12 myotube formation with an increase in protein content, fiber diameter, length, and myo-nuclei was stimulated by lactate. These observations suggest that lactate may be a potential molecule to stimulate muscle hypertrophy and regeneration of mouse skeletal muscle via the activation of muscle satellite cells.

KEYWORDS:

hypertrophy; lactate; muscle satellite cell; regeneration; skeletal muscle

PMID:
30999708
PMCID:
PMC6520919
DOI:
10.3390/nu11040869
[Indexed for MEDLINE]
Free PMC Article

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