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Molecules. 2019 Apr 17;24(8). pii: E1514. doi: 10.3390/molecules24081514.

Overexpression of the Melatonin Synthesis-Related Gene SlCOMT1 Improves the Resistance of Tomato to Salt Stress.

Liu DD1, Sun XS2, Liu L3, Shi HD4, Chen SY5,6,7, Zhao DK8,9,10.

Author information

1
School of Agriculture, Yunnan University, Kunming, Yunnan 650091, China. liudandan@ynu.edu.cn.
2
School of Agriculture, Yunnan University, Kunming, Yunnan 650091, China. sunxiaoshuai2019@163.com.
3
School of Agriculture, Yunnan University, Kunming, Yunnan 650091, China. qiuqiu12-09@163.com.
4
School of Agriculture, Yunnan University, Kunming, Yunnan 650091, China. dong19850412@163.com.
5
Biocontrol Engineering Research Center of Plant Disease & Pest, Yunnan University, Kunming 650504, China. chensuiyun@ynu.edu.cn.
6
Biocontrol Engineering Research Center of Crop Disease & Pest, Yunnan University, Kunming 650504, China. chensuiyun@ynu.edu.cn.
7
School of Life Science, Yunnan University, Kunming 650504, China. chensuiyun@ynu.edu.cn.
8
Biocontrol Engineering Research Center of Plant Disease & Pest, Yunnan University, Kunming 650504, China. zhaodk2012@ynu.edu.cn.
9
Biocontrol Engineering Research Center of Crop Disease & Pest, Yunnan University, Kunming 650504, China. zhaodk2012@ynu.edu.cn.
10
School of Life Science, Yunnan University, Kunming 650504, China. zhaodk2012@ynu.edu.cn.

Abstract

Melatonin can increase plant resistance to stress, and exogenous melatonin has been reported to promote stress resistance in plants. In this study, a melatonin biosynthesis-related SlCOMT1 gene was cloned from tomato (Solanum lycopersicum Mill. cv. Ailsa Craig), which is highly expressed in fruits compared with other organs. The protein was found to locate in the cytoplasm. Melatonin content in SlCOMT1 overexpression transgenic tomato plants was significantly higher than that in wild-type plants. Under 800 mM NaCl stress, the transcript level of SlCOMT1 in tomato leaf was positively related to the melatonin contents. Furthermore, compared with that in wild-type plants, levels of superoxide and hydrogen peroxide were lower while the content of proline was higher in SlCOMT1 transgenic tomatoes. Therefore, SlCOMT1 was closely associated with melatonin biosynthesis confers the significant salt tolerance, providing a clue to cope with the growing global problem of salination in agricultural production.

KEYWORDS:

SlCOMT1; genetical transformation; melatonin; salt stress; tomato

PMID:
30999664
DOI:
10.3390/molecules24081514
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