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Folia Morphol (Warsz). 2019 Apr 17. doi: 10.5603/FM.a2019.0044. [Epub ahead of print]

Reconstruction of lymphatic vessels in the mouse tail after cupping therapy.

Author information

1
Department of Anatomy and Physiology, Shandong College of Traditional Chinese Medicine, Yantai, China. 13853573075@163.com.
2
Department of Anatomy and Physiology, Shandong College of Traditional Chinese Medicine, Yantai, China.
3
Biological Science and Technology Institute, Weifang Medical University, Weifang, China.
4
Department of Breast and Thyroid Surgery, Yantai Affiliated Hospital, Binzhou Medical University, Yantai, China.

Abstract

BACKGROUND:

To investigate the regulatory mechanism of local lymphatic reconstruction after cupping therapy in a mouse model.

MATERIALS AND METHODS:

The lymphatic reconstruction process in the mouse tail after cupping therapy as well as the expression levels of the vascular endothelial identification molecule CD34, prospero homeobox protein 1 (PROX1), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) were investigated for a duration of 4 days through immunohistochemistry experiments.

RESULTS:

On day 1 after cupping therapy, the CD34+ and LYVE-1+ cell densities were significantly increased, and the formed CD34+LYVE-1+ tubular structure started to express PROX1. This was followed by a decrease in both the CD34+ and LYVE-1+ stem cell densities to basal levels on the second day after cupping therapy. Both the CD34+ and LYVE-1+ cell densities subsequently increased again on the third day after cupping therapy. The increase in the LYVE-1+ density was accompanied by tubular structure formation, which is characteristic of lymphangiogenesis. In addition, the colocalization of CD34+ and LYVE-1+ cells by immunohistochemistry suggests that the CD34+ stem cells differentiated into new lymphatic endothelial cells.

CONCLUSIONS:

Our findings indicate that the mechanism underlying the therapeutic effect of cupping therapy involves upregulation of vascular and lymphatic endothelial markers (CD34+, LYVE-1+, and CD34+LYVE-1+) in local tissues, which in turn promotes local new lymphatic vessel formation through the expression of PROX1.

KEYWORDS:

CD34; Cupping; LYVE-1; PROX1; lymphatic regeneration; mice

PMID:
30993665
DOI:
10.5603/FM.a2019.0044
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