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J Cytol. 2019 Apr-Jun;36(2):116-117. doi: 10.4103/JOC.JOC_110_18.

DNA Extraction and Polymerase Chain Reaction.

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Department of Cytology and Gynaecological Pathology, Postgraduate Institute of Medical Education, Chandigarh, India.


DNA extraction and polymerase chain reaction (PCR) are the basic techniques employed in the molecular laboratory. This short overview covers various physical and chemical methods used for DNA extraction so as to obtain a good-quality DNA in sufficient quantity. DNA can be amplified with the help of PCR. The basic principle and different variants of PCR are discussed.


DNA extraction; Polymerase chain reaction; real time PCR

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