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Development. 2019 Apr 16;146(8). pii: dev166173. doi: 10.1242/dev.166173.

Biologically inspired approaches to enhance human organoid complexity.

Author information

1
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
2
Department of Biomedical Engineering, University of Michigan College of Engineering, Ann Arbor, MI 48109, USA.
3
Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA spencejr@umich.edu.
4
Department of Internal Medicine, Division of Gastroenterology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
5
Center for Organogenesis, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Abstract

Organoids are complex three-dimensional in vitro organ-like model systems. Human organoids, which are derived from human pluripotent stem cells or primary human donor tissue, have been used to address fundamental questions about human development, stem cell biology and organ regeneration. Focus has now shifted towards implementation of organoids for biological discovery and advancing existing systems to more faithfully recapitulate the native organ. This work has highlighted significant unknowns in human biology and has invigorated new exploration into the cellular makeup of human organs during development and in the adult - work that is crucial for providing appropriate benchmarks for organoid systems. In this Review, we discuss efforts to characterize human organ cellular complexity and attempts to make organoid models more realistic through co-culture, transplantation and bioengineering approaches.

KEYWORDS:

Human development; Organoids; Stem cells

PMID:
30992275
PMCID:
PMC6503984
[Available on 2020-04-15]
DOI:
10.1242/dev.166173
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