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Fish Shellfish Immunol. 2019 Jun;89:586-594. doi: 10.1016/j.fsi.2019.04.039. Epub 2019 Apr 13.

Effects of microcystin-LR on the immune dysfunction and ultrastructure of hepatopancreas in giant freshwater prawn Macrobrachium rosenbergii.

Author information

1
College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
2
Lake Gaobaoshaobo Fishery Administrative Committee, 732 Middle Yangzijiang Road, Yangzhou, 225009, China.
3
College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address: huiyang@yzu.edu.cn.

Abstract

Microcystins (MCs), produced by cyanobacteria, can strongly inhibit the activity of protein phosphatase, and exhibit strong hepatotoxicity. Macrobrachium rosenbergii is an important aquaculture economic species. Cyanobacterial blooms occur frequently during the culture of M. rosenbergii. However, the effects of MCs on the M. rosenbergii immune function have not been studied. In the present study, M. rosenbergii were exposed to environment-related concentrations of MC-LR type (0.5 and 5 μg/L) for 3 weeks. Hepatopancreatic histology was investigated, and antioxidant enzymes activity, acid phosphatase, alkaline phosphatase and lysozyme activity in hepatopancreas were also analyzed. Results showed that MC-LR could damage M. rosenbergii hepatopancreas, induce hepatopancreatic apoptosis and antioxidant dysfunctions. The expression profiles of major immune-related genes after MC-LR exposure were also detected. Some genes with antibacterial functions were suppressed, and the expression of apoptosis-related genes were up-regulated. After MC-LR exposure, the cumulative mortality of M. rosenbergii infected with Vibrio vulnificus and Aeromonas hydrophila were much higher than the control in a time-dose dependent manner. These results indicated the potential negative influence of MC-LR on the immune function of M. rosenbergii.

KEYWORDS:

Bacteria; Hepatopancreas; Immune response; Macrobrachium rosenbergii; Microcystin-LR

PMID:
30991147
DOI:
10.1016/j.fsi.2019.04.039

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