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J Cell Mol Med. 2019 Apr 15. doi: 10.1111/jcmm.14296. [Epub ahead of print]

Alpha lipoic acid inhibits oxidative stress-induced apoptosis by modulating of Nrf2 signalling pathway after traumatic brain injury.

Author information

1
Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui Province, China.
2
Department of Neurosurgery, Huai'an Second People's Hospital, Xuzhou Medical College, Huai'an, Jiangsu Province, China.
3
Department of Radiology, Huai'an Fourth people's Hospital, Huai'an, Jiangsu Province, China.
4
Department of Anesthesiology, the Second Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China.
5
Department of Neurosurgery, Jiangsu Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province, China.

Abstract

Alpha lipoic acid (ALA) is a powerful antioxidant which has been widely used in the treatment of different system diseases, such as cardiovascular and cerebrovascular diseases. But, there are few studies that refer to protective effects and potential mechanisms on traumatic brain injury (TBI). This study was carried out to investigate the neuroprotective effect following TBI and illuminate the underlying mechanism. Weight drop-injured model in rats was induced by weight-drop. ALA was administrated via intraperitoneal injection after TBI. Neurologic scores were examined following several tests. Neurological score was performed to measure behavioural outcomes. Nissl staining and TUNEL were performed to evaluate the neuronal apoptosis. Western blotting was engaged to analyse the protein content of the Nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream protein factors, including hemeoxygenase-1 (HO-1) and quinine oxidoreductase-1 (NQO1). ALA treatment alleviated TBI-induced neuron cell apoptosis and improved neurobehavioural function by up-regulation of Nrf2 expression and its downstream protein factors after TBI. This study presents new perspective of the mechanisms responsible for the neuronal apoptosis of ALA, with possible involvement of Nrf2 pathway.

KEYWORDS:

ALA; Nrf2; neuronal apoptosis; traumatic brain injury

PMID:
30989783
DOI:
10.1111/jcmm.14296
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