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Molecules. 2019 Apr 2;24(7). pii: E1294. doi: 10.3390/molecules24071294.

Towards Understanding of Polymorphism of the G-rich Region of Human Papillomavirus Type 52.

Author information

1
Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia. marusic.maja@ki.si.
2
Slovenian NMR Center, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia. janez.plavec@ki.si.
3
EN-FIST Center of Excellence, SI-1000 Ljubljana, Slovenia. janez.plavec@ki.si.
4
Faculty of Chemistry and Chemical Technology, University of Ljubljana, SI-1000 Ljubljana, Slovenia. janez.plavec@ki.si.

Abstract

The potential to affect gene expression via G-quadruplex stabilization has been extended to all domains of life, including viruses. Here, we investigate the polymorphism and structures of G-quadruplexes of the human papillomavirus type 52 with UV, CD and NMR spectroscopy and gel electrophoresis. We show that oligonucleotide with five G-tracts folds into several structures and that naturally occurring single nucleotide polymorphisms (SNPs) have profound effects on the structural polymorphism in the context of G-quadruplex forming propensity, conformational heterogeneity and folding stability. With help of SNP analysis, we were able to select one of the predominant forms, formed by G-rich sequence d(G₃TAG₃CAG₄ACACAG₃T). This oligonucleotide termed HPV52(1-4) adopts a three G-quartet snap back (3 + 1) type scaffold with four syn guanine residues, two edgewise loops spanning the same groove, a no-residue V loop and a propeller type loop. The first guanine residue is incorporated in the central G-quartet and all four-guanine residues from G4 stretch are included in the three quartet G-quadruplex core. Modification studies identified several structural elements that are important for stabilization of the described G-quadruplex fold. Our results expand set of G-rich targets in viral genomes and address the fundamental questions regarding folding of G-rich sequences.

KEYWORDS:

DNA; G-quadruplex; NMR; folding; human papillomaviruses; structure

PMID:
30987050
PMCID:
PMC6479982
DOI:
10.3390/molecules24071294
[Indexed for MEDLINE]
Free PMC Article

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