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J Vis Exp. 2019 Mar 29;(145). doi: 10.3791/59235.

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression.

Author information

1
Center for Epigenetics, Van Andel Research Institute.
2
Amgen.
3
Center for Epigenetics, Van Andel Research Institute; KwangHo.Lee@vai.org.

Abstract

Here we describe a protocol for implementing the REMOTE-control system (Reversible Manipulation of Transcription at Endogenous loci), which allows for reversible and tunable expression control of an endogenous gene of interest in living model systems. The REMOTE-control system employs enhanced lac repression and tet activation systems to achieve down- or upregulation of a target gene within a single biological system. Tight repression can be achieved from repressor binding sites flexibly located far downstream of a transcription start site by inhibiting transcription elongation. Robust upregulation can be attained by enhancing the transcription of an endogenous gene by targeting tet transcriptional activators to the cognate promoter. This reversible and tunable expression control can be applied and withdrawn repeatedly in organisms. The potency and versatility of the system, as demonstrated for endogenous Dnmt1 here, will allow more precise in vivo functional analyses by enabling investigation of gene function at various expression levels and by testing the reversibility of a phenotype.

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