Format

Send to

Choose Destination
Adv Healthc Mater. 2019 Apr 15:e1900167. doi: 10.1002/adhm.201900167. [Epub ahead of print]

A Versatile Biocompatible Antibiotic Delivery System Based on Self-Assembling Peptides with Antimicrobial and Regenerative Potential.

Author information

1
School of Life Sciences, Institute for Chemistry and Bioanalytics, University of Applied Sciences and Arts Northwestern Switzerland, 4132, Muttenz, Switzerland.
2
Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057, Rostock, Germany.
3
Department of Cell Biology, University Medicine Rostock, 18057, Rostock, Germany.
4
Clinic for Restorative Dentistry and Periodontology, University Medicine Rostock, 18057, Rostock, Germany.
5
School of Life Sciences, Institute of Pharma Technology, University of Applied Sciences and Arts Northwestern Switzerland, 4132, Muttenz, Switzerland.

Abstract

Periodontitis is a chronic inflammatory and tissue-destructive disease. Since the polymicrobiome in the oral cavity makes it difficult to treat, novel therapeutic strategies are required. Hydrogels based on self-assembling peptides (SAP) can be suitable candidates for periodontal therapy due to their injectability, biocompatibility, cargo-loading capacity, and tunable physicochemical and mechanical properties. In this study, two SAP hydrogels (P11-4 and P11-28/29) are examined for their intrinsic antimicrobial activity, regenerative potential, and antibiotic delivery capacity. A significant antibacterial effect of P11-28/29 hydrogels on the periodontal pathogen Porphyromonas gingivalis and a less pronounced effect for P11-4 hydrogels is demonstrated. The metabolic activity rates of human dental follicle stem cells (DFSCs), which reflect cell viability and may thus indicate the regenerative capacity, are similar on tissue culture polystyrene (TCPS) and on P11-4 hydrogels after 14 days of culture. Noticeably, both SAP hydrogels strengthen the osteogenic differentiation of DFSCs compared with TCPS. The incorporation of tetracycline, ciprofloxacin, and doxycycline does not affect fibril formation of either SAP hydrogel and results in favorable release kinetics up to 120 h. In summary, this study reveals that P11-SAP hydrogels combine many favorable properties required to make them applicable as prospective novel treatment strategy for periodontal therapy.

KEYWORDS:

antibiotic delivery; antimicrobial activity; dental stem cells; periodontal disease; self-assembling peptides

PMID:
30985084
DOI:
10.1002/adhm.201900167

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center