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J Funct Foods. 2018 Nov;50:1-7. doi: 10.1016/j.jff.2018.09.021. Epub 2018 Sep 25.

Theaflavin-3, 3'-digallate inhibits ovarian cancer stem cells via suppressing Wnt/β-Catenin signaling pathway.

Author information

1
Department of Tea Science, Zhejiang University, Hangzhou, Zhejiang, P.R. China.
2
College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV , USA.
3
Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, USA.
4
Department of Pharmaceutical Sciences, West Virginia University, Morgantown, WV, USA.
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Contributed equally

Abstract

Recent evidence indicates that ovarian cancer stem cells (CSCs) are responsible for ovarian cancer recurrence and drug resistance, resulting in the low long-term survival rate of patients with advanced ovarian cancer. We aimed to study the inhibitory effect of theaflavin-3, 3'-digallate (TF3), a black tea polyphenol on ovarian CSCs. Here, we showed that TF3 inhibited the proliferation of A2780/CP70 and OVCAR3 tumorshpere cells by suppressing their cell viability and colony formation capacity. TF3 inhibited the tumorsphere formation capacity of A2780/CP70 and OVCAR3 CSCs in serum-free and non-adherent conditions. TF3 inhibited A2780/CP70 and OVCAR3 CSCs isolated from tumorspheres by decreasing their cell viability and upregulating the protein expression of caspase-3 and -7 in the cells. We also revealed that TF3 inhibited ovarian CSCs through Wnt/β-catenin signaling pathway. Our results suggested that TF3 could inhibit ovarian CSCs and might be a potential agent for eradicating ovarian cancer.

KEYWORDS:

3’-digallate; ALDH; anti-proliferation; ovarian cancer stem cell; theaflavin-3; β-Catenin

PMID:
30984291
PMCID:
PMC6456259
[Available on 2019-11-01]
DOI:
10.1016/j.jff.2018.09.021

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