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J Periodontal Res. 2019 Apr 15. doi: 10.1111/jre.12657. [Epub ahead of print]

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice.

Author information

1
Department of Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
2
Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
3
Pre-Major of Cosmetics and Pharmaceutics, Daegu Haany University, Gyeongsan, Korea.
4
Department of Oral Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu, South Korea.
5
Department of Conservative Dentistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
6
Department of Oral and Maxillofacial Surgery, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
7
Department of Periodontology, School of Dentistry, IHBR, Kyungpook National University, Daegu, Korea.
8
Department of Dental Hygiene, College of Health Science, Gachon University, Incheon, Korea.
9
Immunoregulatory Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Korea.
10
Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Korea.
11
Department of Histology and Developmental Biology, Tokyo Dental College, Tokyo, Japan.
12
Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Korea.

Abstract

OBJECTIVE:

We evaluated the role of oleanolic acid acetate (OAA), a triterpenoid commonly used in the treatment of liver disorders, inflammatory diseases, and metastasis, in bone formation after tooth loss by periodontitis.

BACKGROUND:

Periodontitis causes the sequential degradation of the alveolar bone and associated structures, resulting in tooth loss. Several studies have attempted to regenerate the bone for implantation following tooth loss.

METHODS:

Maxillary left second molar was extracted from 8-week-old male mice following induction of periodontitis by ligature for 5 days. The extraction socket was treated with 50 ng/µL OAA for 1, 2, and 3 weeks. Detailed morphological changes were examined using Masson's trichrome staining, and the precise localization patterns of various signaling molecules, including CD31, F4/80, interleukin (IL)-6, and osteocalcin, were observed. The volume of bone formation was examined by Micro-CT. Osteoclasts were enumerated using tartrate-resistant acid phosphatase (TRAP) staining. For molecular dissection of signaling molecules, we employed the hanging-drop in vitro cultivation method at E14 for 1 day and examined the expression pattern of transforming growth factor (TGF)-β superfamily and Wnt signaling genes.

RESULTS:

Histomorphometrical examinations showed facilitated bone formation in the extraction socket following OAA treatment. In addition, OAA-treated specimens showed the altered localization patterns of inflammatory and bone formation-related signaling molecules including CD31, F4/80, IL-6, and osteocalcin. Also, embryonic tooth germ mesenchymal tissue cultivation with OAA treatment showed the significant altered expression patterns of signaling molecules such as transforming growth factor (TGF)-β superfamily and Wnt signaling.

CONCLUSIONS:

Oleanolic acid acetate induces bone formation and remodeling through proper modulation of osteoblast, osteoclast, and inflammation with regulations of TGF-β and Wnt signaling.

KEYWORDS:

alveolar bone; chronic periodontitis; inflammation; regeneration

PMID:
30982986
DOI:
10.1111/jre.12657

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