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Mol Cell Proteomics. 2019 Apr 12. pii: mcp.RA118.001101. doi: 10.1074/mcp.RA118.001101. [Epub ahead of print]

Integrated transcriptomic and proteomic analysis of human eccrine sweat glands identifies missing and novel proteins.

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Johns Hopkins University School of Medicine, United States of America.
University of Maryland.
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine.
Laboratory Medicine and Pathology, Mayo Clinic, United States of America


The eccrine sweat gland is an exocrine gland that is involved in the secretion of sweat for control of temperature. Malfunction of the sweat glands can result in disorders such as miliaria, hyperhidrosis and bromhidrosis. Understanding the transcriptome and proteome of sweat glands is important for understanding their physiology and role in diseases. However, no systematic transcriptome or proteome analysis of sweat glands has yet been reported. Here, we isolated eccrine sweat glands from human skin by microdissection and performed RNA-seq and proteome analysis. In total, ~138,000 transcripts and ~6,100 proteins were identified. Comparison of the RNA-seq data of eccrine sweat glands to other human tissues revealed the closest resemblance to the cortex region of kidneys. The proteome data showed enrichment of proteins involved in secretion, reabsorption, and wound healing. Importantly, protein level identification of the calcium ion channel TRPV4 suggests the importance of eccrine sweat glands in re-epithelialization of wounds and prevention of dehydration. We also identified 2 previously missing proteins from our analysis. Using a proteogenomic approach, we identified 7 peptides from 5 novel genes, which we validated using synthetic peptides. Most of the novel proteins were from short open reading frames (sORFs) suggesting that many sORFs still remain to be annotated in the human genome. This study presents the first integrated analysis of the transcriptome and proteome of the human eccrine sweat gland and would become a valuable resource for studying sweat glands in physiology and disease.


Cystic fibrosis; Mass Spectrometry; Peptide Synthesis*; Proteogenomics; Proteomics; RNA SEQ; Sweat glands; Transcriptomics

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