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J Microbiol Methods. 2019 Jun;161:35-42. doi: 10.1016/j.mimet.2019.04.007. Epub 2019 Apr 9.

Rapid detection of heavy metal-induced toxicity in water using a fed-batch sulfur-oxidizing bacteria (SOB) bioreactor.

Author information

1
Department of Biological Environment, Kangwon National University, 192-1 Hyoja-dong, Gangwon-do, Chuncheon-si 200-701, Republic of Korea.
2
Botany and Microbiology Department, Faculty of Science, New Valley University, El-Kharga 72511, Egypt.
3
Department of Environmental Engineering, Kangwon National University, 192-1 Hyoja-dong, Gangwon-do, Chuncheon-si 200-701, Republic of Korea.
4
Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul 04763, Republic of Korea.
5
Department of Environmental Engineering, Korea Maritime and Ocean University, Busan 49112, Republic of Korea.
6
Department of Biological Environment, Kangwon National University, 192-1 Hyoja-dong, Gangwon-do, Chuncheon-si 200-701, Republic of Korea. Electronic address: ohsangeun@kangwon.ac.kr.

Abstract

A fed-batch bioreactor based on sulfur-oxidizing bacteria (SOB) was tested for rapid detection of heavy metal-induced toxicity in water. For this evaluation, SOB were exposed to water contaminated by selenium, mercury, hexavalent chromium, arsenic, cyanide, cadmium, and lead for 2 h and their inhibition rates were analyzed based on changes in electrical conductivity (EC). The results demonstrate that SOB were highly inhibited by selenium, mercury, hexavalent chromium, and arsenic but not by cyanide, cadmium, and lead. The 2 h half maximum effective concentrations (EC50) of SOB for selenium, mercury, hexavalent chromium, and arsenic were estimated to be 0.33, 0.89, 1.18, and 0.24 mg/L, respectively, which are comparable or lower than earlier reports in the literature. However, the EC50 or EC20 values of SOB for cyanide, cadmium, and lead were notably higher compared to findings from previous toxicity tests that employed other microorganisms. The findings from the current study suggest that the fed-batch SOB bioreactor is suitable for rapid detection of toxicity induced by selenium, mercury, hexavalent chromium, and arsenic in water.

KEYWORDS:

Electrical conductivity; Fed-batch bioreactor; Heavy metals; Sulfur-oxidizing bacteria; Toxicity test

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