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Proc Natl Acad Sci U S A. 2019 Apr 30;116(18):8685-8692. doi: 10.1073/pnas.1816835116. Epub 2019 Apr 11.

A diecast mineralization process forms the tough mantis shrimp dactyl club.

Author information

1
Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University (NTU), 639798 Singapore.
2
School of Materials Science and Engineering, NTU, 639798 Singapore.
3
Life Science Institutes, Singapore Lipidomics Incubator, National University of Singapore (NUS), 117456 Singapore.
4
Functional Proteomics Laboratory, Institute for Molecular, Cell, and Development Biology, Agency for Science, Technology, and Research (A*Star), 138673 Proteos, Singapore.
5
Molecular Engineering Laboratory, Biomedical Sciences Institutes, A*Star, 138673 Proteos, Singapore.
6
Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University (NTU), 639798 Singapore; ali.miserez@ntu.edu.sg.
7
School of Biological Sciences, NTU, 637551 Singapore.

Abstract

Biomineralization, the process by which mineralized tissues grow and harden via biogenic mineral deposition, is a relatively lengthy process in many mineral-producing organisms, resulting in challenges to study the growth and biomineralization of complex hard mineralized tissues. Arthropods are ideal model organisms to study biomineralization because they regularly molt their exoskeletons and grow new ones in a relatively fast timescale, providing opportunities to track mineralization of entire tissues. Here, we monitored the biomineralization of the mantis shrimp dactyl club-a model bioapatite-based mineralized structure with exceptional mechanical properties-immediately after ecdysis until the formation of the fully functional club and unveil an unusual development mechanism. A flexible membrane initially folded within the club cavity expands to form the new club's envelope. Mineralization proceeds inwards by mineral deposition from this membrane, which contains proteins regulating mineralization. Building a transcriptome of the club tissue and probing it with proteomic data, we identified and sequenced Club Mineralization Protein 1 (CMP-1), an abundant mildly phosphorylated protein from the flexible membrane suggested to be involved in calcium phosphate mineralization of the club, as indicated by in vitro studies using recombinant CMP-1. This work provides a comprehensive picture of the development of a complex hard tissue, from the secretion of its organic macromolecular template to the formation of the fully functional club.

KEYWORDS:

bioapatite; biomineralization; ecdysis; mineralization proteins; stomatopod dactyl club

PMID:
30975751
PMCID:
PMC6500109
[Available on 2019-10-11]
DOI:
10.1073/pnas.1816835116

Conflict of interest statement

The authors declare no conflict of interest.

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