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Vet Res. 2019 Apr 11;50(1):25. doi: 10.1186/s13567-019-0643-1.

Bovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureus.

Author information

1
Department of Animal Production Science, Faculty of Veterinary Medicine, University of Chile, 8820808, Santiago, Chile.
2
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, 24060, USA.
3
Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Sciences, Austral University of Chile, 5110566, Valdivia, Chile.
4
Institute of Dental Science Research, Faculty of Dentistry, University of Chile, 8380492, Santiago, Chile.
5
Department of Preventive Medicine, Faculty of Veterinary Medicine, University of Chile, 8820808, Santiago, Chile.
6
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Chile, 8820808, Santiago, Chile.
7
Department of Animal Production Science, Faculty of Veterinary Medicine, University of Chile, 8820808, Santiago, Chile. operalta@uchile.cl.
8
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA, 24060, USA. operalta@uchile.cl.

Abstract

Staphylococcus aureus is the most commonly isolated pathogen from clinical bovine mastitis samples and a difficult pathogen to combat. Mesenchymal stem cells (MSC) are multipotent progenitor cells equipped with a variety of factors that inhibit bacterial growth. The aim of the present study was to evaluate the in vitro antibacterial potential against S. aureus of conditioned medium (CM) from MSC derived from fetal bovine bone marrow (BM-MSC) and adipose tissue (AT-MSC). BM-MSC, AT-MSC and fetal fibroblasts (FB) cultures were activated by infection with S. aureus. Bacterial growth was evaluated in presence of CM, concentrated CM (CCM), activated CM (ACM) and concentrated ACM (CACM) from BM-MSC, AT-MSC and FB. Gene expression of β-defensin 4A (bBD-4A), NK-lysine 1 (NK1), cathelicidin 2 (CATHL2), hepcidin (HEP) and indoleamine 2,3 dioxygenase (IDO) and protein expression of bBD-4A were determined in activated and non-activated cells. The majority of BM-MSC and AT-MSC expressed CD73, Oct4 and Nanog, and were negative for CD34. Growth of S. aureus decreased when it was exposed to CM from BM-MSC, AT-MSC and FB. Moreover, growth of S. aureus in CCM, ACM and CACM was lower compared to controls of CM from BM-MSC and AT-MSC. Activated AT-MSC increased mRNA levels of bBD4A and NK1, and protein levels of bBD4A in CM. Thus, CM from fetal bovine BM-MSC and AT-MSC has the capacity to reduce in average ~30% of S. aureus relative growth under in vitro conditions. The in vitro antibacterial effect of fetal bovine MSC may be mediated by bBD4A and NK1 activity.

PMID:
30975214
PMCID:
PMC6460550
DOI:
10.1186/s13567-019-0643-1
[Indexed for MEDLINE]
Free PMC Article

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