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Toxins (Basel). 2019 Apr 9;11(4). pii: E214. doi: 10.3390/toxins11040214.

Fluorescence Spectroscopic Investigation of Competitive Interactions between Quercetin and Aflatoxin B₁ for Binding to Human Serum Albumin.

Tan H1, Chen L2, Ma L3,4, Liu S5, Zhou H6, Zhang Y7,8, Guo T9, Liu W10, Dai H11, Yu Y12.

Author information

1
College of Food Science, Southwest University, Chongqing 400715, China. tanhongxia@mail.swu.edu.cn.
2
College of Food Science, Southwest University, Chongqing 400715, China. chenluch@email.swu.edu.cn.
3
College of Food Science, Southwest University, Chongqing 400715, China. zhyhml@swu.edu.cn.
4
Biological Science Research Center, Southwest University, Chongqing 400715, China. zhyhml@swu.edu.cn.
5
College of Food Science, Southwest University, Chongqing 400715, China. liushuang9995@stu.ouc.edu.cn.
6
College of Food Science, Southwest University, Chongqing 400715, China. zhouhy@swu.edu.cn.
7
College of Food Science, Southwest University, Chongqing 400715, China. zhy1203@swu.edu.cn.
8
Biological Science Research Center, Southwest University, Chongqing 400715, China. zhy1203@swu.edu.cn.
9
College of Food Science, Southwest University, Chongqing 400715, China. guoting06@swu.edu.cn.
10
College of Food Science, Southwest University, Chongqing 400715, China. lwissue@email.swu.cn.
11
College of Food Science, Southwest University, Chongqing 400715, China. daihongjie@swu.edu.cn.
12
College of Food Science, Southwest University, Chongqing 400715, China. yuyong@swu.edu.cn.

Abstract

Aflatoxin B1 (AFB₁) is a highly toxic mycotoxin found worldwide in cereals, food, and animal feeds. AFB₁ binds to human serum albumin (HSA) with high affinity. In previous experiments, it has been revealed that reducing the binding rate of AFB₁ with HSA could speed up the elimination rate of AFB₁. Therefore, we examined the ability of quercetin to compete with AFB₁ for binding HSA by fluorescence spectroscopy, synchronous spectroscopy, ultrafiltration studies, etc. It was shown that AFB₁ and quercetin bind to HSA in the same Sudlow site Ӏ (subdomain IIA), and the binding constant (Ka) of the quercetin-HSA complex is significantly stronger than the complex of AFB₁-HSA. Our data in this experiment showed that quercetin is able to remove the AFB₁ from HSA and reduce its bound fraction. This exploratory work may be of significance for studies in the future regarding decreasing its bound fraction and then increasing its elimination rate for detoxification. This exploratory study may initiate future epidemiological research designs to obtain further in vivo evidence of the long-term (potential protective) effects of competing substances on human patients.

KEYWORDS:

aflatoxin B1; competitive interaction; fluorescence spectroscopy; human serum albumin; quercetin

Conflict of interest statement

The funders had no role in the design of the study; in the collection, analyses, or interpretation of the data; in the writing of the manuscript; nor in the decision to publish the results.

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