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Cytotechnology. 2019 Jun;71(3):679-686. doi: 10.1007/s10616-019-00313-6. Epub 2019 Apr 9.

Cubeben induces autophagy via PI3K-AKT-mTOR pathway to protect primary neurons against amyloid beta in Alzheimer's disease.

Author information

1
Department of Neurology, ZiBo Central Hospital, 54 Communist Youth League West Road, Zibo, 255036, Shandong Province, China.
2
Department of Neurology, ZiBo Central Hospital, 54 Communist Youth League West Road, Zibo, 255036, Shandong Province, China. DenisetPerezev@yahoo.com.

Abstract

Autophagy is a lysosomal degradative process by which it recycles cytosolic components and degrades protein aggregates inside cells. Here, we identified cubeben as an inducer of autophagy in primary neuronal cells. Autophagy induction was accompanied the upregulation of autophagic proteins like Beclin, ATG5, ATG12 and lipidation of LC3-II in primary neuronal cells. Cubeben induces autophagy by the inhibition of PI3K-AKT pathway in a dose dependent manner. Constitutive active P110α abrogates cubeben induced autophagic induction in primary neuronal cells. Furthermore, cubeben inhibits amyloid beta induced toxicity in primary neuronal cells. Thus our data suggests that cubeben as a potential anti-Alzheimer therapeutic lead.

KEYWORDS:

Alzheimer’s disease; Amyloid beta; Autophagy; Neuroprotection; Primary neuronal cells

PMID:
30968233
PMCID:
PMC6546769
[Available on 2020-06-01]
DOI:
10.1007/s10616-019-00313-6

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