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Biochem Biophys Res Commun. 2019 Apr 6. pii: S0006-291X(19)30595-9. doi: 10.1016/j.bbrc.2019.03.188. [Epub ahead of print]

Antibodies against H1N1 influenza virus hemagglutinin cross-react with prohibitin.

Author information

1
Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, China; Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China.
2
Institute of Basic and Translational Medicine, Xi'an Medical University, Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Xi'an, Shaanxi, China.
3
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi'an, Shaanxi, China; Department of Translational Medicine, Institute of Integrated Medical Information, Xi'an, Shaanxi, China. Electronic address: xiexin@nwu.edu.cn.
4
Central Laboratory of Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, China; Research Center of Cell Immunological Engineering and Technology of Shaanxi Province, Xi'an, Shaanxi, China. Electronic address: hujun_1112@126.com.

Abstract

Influenza virus infection is associated with type 1 diabetes (T1DM), but its pathogenesis remains unclear. Here, our study found that one of the monoclonal antibodies against H1N1 influenza virus hemagglutinin(HA) cross-reacted with human pancreatic tissue and further demonstrated that it binded to rat islet β-cells. We immunoprecipitated islet protein with this cross-reactive antibody and identified the bound antigen as prohibitin by mass spectrometry. We then expressed the prohibitin protein in bacteria and confirmed the antibody binding to prohibitin by Western blot. We also verified the cross-reactivity of the antibody by prohibitin-siRNA transfection in islet beta cells. We conclude that prohibitin is an autoantigen that cross-reacts with influenza virus HA. The correlation between the autoantigen prohibitin and type 1 diabetes remains to be investigated.

KEYWORDS:

Cross-reactivity; Influenza virus; Type 1 diabetes

PMID:
30967257
DOI:
10.1016/j.bbrc.2019.03.188

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