Cryo-EM Structure of a Begomovirus Geminate Particle

Xiongbiao Xu; Qing Zhang; Jian Hong; Zhenghe Li; Xiaokang Zhang; Xueping Zhou

doi:10.3390/ijms20071738

Cryo-EM Structure of a Begomovirus Geminate Particle

Int J Mol Sci. 2019 Apr 8;20(7):1738. doi: 10.3390/ijms20071738.

Authors

Xiongbiao Xu^{1

2}, Qing Zhang^{3

4}, Jian Hong⁵, Zhenghe Li⁶, Xiaokang Zhang^{7

8}, Xueping Zhou^{9

10}

Affiliations

¹ State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. xiongbiaox1988@126.com.
² Guangxi Key Laboratory of Medicinal Resources Conservation and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China. xiongbiaox1988@126.com.
³ Department of Biophysics, School of Medicine, Zhejiang University, Hangzhou 310058, China. 1618557@zju.edu.cn.
⁴ Center of Cryo Electron Microscopy, Zhejiang University, Hangzhou 310058, China. 1618557@zju.edu.cn.
⁵ Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China. jhong@zju.edu.cn.
⁶ State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. lizh@zju.edu.cn.
⁷ Department of Biophysics, School of Medicine, Zhejiang University, Hangzhou 310058, China. xzhang1965@zju.edu.cn.
⁸ Center of Cryo Electron Microscopy, Zhejiang University, Hangzhou 310058, China. xzhang1965@zju.edu.cn.
⁹ State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. zzhou@zju.edu.cn.
¹⁰ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. zzhou@zju.edu.cn.

Abstract

Tobacco curly shoot virus, a monopartite begomovirus associated with betasatellite, causes serious leaf curl diseases on tomato and tobacco in China. Using single-particle cryo-electron microscopy, we determined the structure of tobacco curly shoot virus (TbCSV) particle at 3.57 Å resolution and confirmed the characteristic geminate architecture with single-strand DNA bound to each coat protein (CP). The CP⁻CP and DNA⁻CP interactions, arranged in a CP⁻DNA⁻CP pattern at the interface, were partially observed. This suggests the genomic DNA plays an important role in forming a stable interface during assembly of the geminate particle.

Keywords: Cryo-EM; asymmetric reconstruction; geminate capsid; geminivirus; single capsid; single particle analysis.

MeSH terms

Begomovirus / ultrastructure*
Capsid / ultrastructure
Cryoelectron Microscopy / methods*
DNA, Viral / ultrastructure

Substances

DNA, Viral

Supplementary concepts

Tobacco curly shoot virus

Abstract

MeSH terms

Substances

Supplementary concepts

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