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Endocr Connect. 2019 Apr 1. pii: EC-18-0537.R2. doi: 10.1530/EC-18-0537. [Epub ahead of print]

A preliminary transcriptome analysis suggests a transitory effect of vitamin D on mitochondrial function in obese young Finnish subjects.

Author information

1
E Einarsdottir, Folkhälsan Institute of Genetics, Folkhälsan Research Center, University of Helsinki, Finland.
2
M Pekkinen, Institute of Genetics, Folkhälsan Research Center, University of Helsinki, Finland.
3
K Krjutškov, Molecular Neurology Research Program, Helsingin Yliopisto, Helsinki, Finland.
4
S Katayama, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Finland.
5
J Kere, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
6
O Mäkitie, Pediatric Endocrinology and Metabolic Bone Diseases, Hospital for Children and Adolescents, Helsinki, FIN-00029 HUS, Finland.
7
H Viljakainen, Folkhälsan Institute of Genetics, Folkhälsan Research Center, University of Helsinki, Finland.

Abstract

OBJECTIVE:

The effect of vitamin D at the transcriptome level is poorly understood, and furthermore it is unclear if it differs between obese and normal-weight subjects. We aimed to explore transcriptome effects of vitamin D supplementation.

DESIGN AND METHODS:

We analysed peripheral blood gene expression using GlobinLock oligonucleotides followed by RNA sequencing in individuals participating in a 12-week randomised double-blinded placebo-controlled vitamin D intervention study. The study involved 18 obese and 18 normal-weight subjects (of which 20 males) with mean (±SD) age 20.4 (±2.5) years and BMIs 36 (±10) and 23 (±4) kg/m2, respectively. The supplemental daily vitamin D dose was 50 µg (2000 IU). Data was available at baseline, 6- and 12-week time points and comparisons were performed between the vitamin D and placebo groups separately in obese and normal-weight subjects.

RESULTS:

Significant transcriptomic changes were observed at 6 weeks, and only in the obese subjects: 1724 genes were significantly upregulated and 186 genes were downregulated in the vitamin D group compared with placebo. Further analyses showed several enriched gene categories connected to mitochondrial function and metabolism, and the most significantly enriched pathway was related to oxidative phosphorylation (adjusted p-value 3.08×10-14). Taken together, our data suggest an effect of vitamin D supplementation on mitochondrial function in obese subjects.

CONCLUSIONS:

Vitamin D supplementation affects gene expression in obese, but not in normal-weight subjects. The altered genes are enriched in pathways related to mitochondrial function. The present study increases the understanding of the effects of vitamin D at the transcriptome level.

PMID:
30965285
DOI:
10.1530/EC-18-0537
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