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Molecules. 2019 Apr 5;24(7). pii: E1344. doi: 10.3390/molecules24071344.

Regulation of Noise-Induced Loss of Serotonin Transporters with Resveratrol in a Rat Model Using 4-[18F]-ADAM/Small-Animal Positron Emission Tomography.

Author information

1
Department of Pharmacy Practice, Tri-Service General Hospital, Taipei 114, Taiwan. lhs01077@gmail.com.
2
School of Pharmacy, National Defense Medical Center, Taipei 114, Taiwan. lhs01077@gmail.com.
3
Department of Pharmacy Practice, Tri-Service General Hospital, Taipei 114, Taiwan. jtlovehl@gmail.com.
4
School of Pharmacy, National Defense Medical Center, Taipei 114, Taiwan. jtlovehl@gmail.com.
5
Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan. ytcola581470@gmail.com.
6
Department of Otorhinolaryngology-Head and Neck Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan. acolufreia@yahoo.com.tw.
7
Department of Nuclear Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan. treasure316@gmail.com.
8
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan. t70cyy@yahoo.com.
9
Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan. anatoman2001@yahoo.com.tw.
10
Department of Nuclear Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan. shiue@ntuh.gov.tw.
11
Department of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan. kuohsing91@yahoo.com.tw.

Abstract

Serotonin (5-HT) plays a crucial role in modulating the afferent fiber discharge rate in the inferior colliculus, auditory cortex, and other nuclei of the ascending auditory system. Resveratrol, a natural polyphenol phytoalexin, can inhibit serotonin transporters (SERT) to increase synaptic 5-HT levels. In this study, we investigated the effects of resveratrol on noise-induced damage in the serotonergic system. Male Sprague-Dawley rats were anaesthetized and exposed to an 8-kHz tone at 116 dB for 3.5 h. Resveratrol (30 mg/kg, intraperitoneal injection [IP]) and citalopram (20 mg/kg, IP), a specific SERT inhibitor used as a positive control, were administered once a day for four consecutive days, with the first treatment occurring 2 days before noise exposure. Auditory brainstem response testing and positron emission tomography (PET) with N,N-dimethyl-2-(2-amino-4-[18F]fluorophenylthio)benzylamine (4-[18F]-ADAM, a specific radioligand for SERT) were used to evaluate functionality of the auditory system and integrity of the serotonergic system, respectively, before and after noise exposure. Finally, immunohistochemistry was performed 1 day after the last PET scan. Our results indicate that noise-induced serotonergic fiber loss occurred in multiple brain regions including the midbrain, thalamus, hypothalamus, striatum, auditory cortex, and frontal cortex. This noise-induced damage to the serotonergic system was ameliorated in response to treatment with resveratrol and citalopram. However, noise exposure increased the hearing threshold in the rats regardless of drug treatment status. We conclude that resveratrol has protective effects against noise-induced loss of SERT.

KEYWORDS:

4-[18F]-ADAM; noise-induced hearing loss; resveratrol; serotonin transporters; small-animal PET

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